An experimental study is proposed to investigate the high-cycle fatigue behavior of prestressed concrete box-girders. Special focus is on the shear diagonal cracks in the webs of girders, which is rarely investigated in the open literature. Laboratory fatigue tests were designed and conducted to study the propagation of shear diagonal crack of PC box-girder specimens under cyclic loadings. The development of stirrup strain, propagation characteristics of diagonal crack and the related factors are discussed in detail. A finite element (FE) model...