期刊论文

Guo, Z.W.

中文

中药材

1001-4454

2010

33

7

1038-1041

10.13863/j.issn1001-4454.2010.07.002

湖南省科技厅项目（2007FJ4139 04NK3007） 湖南省教育厅项目（08C084）

本校为第一机构

目的:解决野生药用植物黄花石蒜外植体大小影响其组织培养效果的难题.方法:选用其鳞茎的带底盘双内层鳞片,带底盘双中层鳞片为材料,采用长宽为9.0×8.0mm,6.0×5.0mm,3.0×2.0mm3种不同大小的外植体,接种在MS+9mg/LBA+5mg/LNAA+0.7%琼脂+3%蔗糖与MS+5mg/LBA+9mg/LNAA+0.7%琼脂+3%蔗糖2种培养基上进行培养,研究了黄花石蒜外植体的不同大小对其组织培养效果的影响.结果与结论:6.0×5.0mm中等大小的带底盘双内层鳞片外植体在MS+9mg/LBA+5mg/LNAA+0.7%琼脂+3%蔗糖的培养基上培养有利于不定芽,不定根的发生,产生褐变量也较少.相关分析表明,不同大小的2种外植体在2种培养基上的培养效果为极显著正相关...

OBJECTIVE: To solve the puzzle about the right size of the explant of wild medicinal plant Lycoris aurea for tissue culture. METHODS: Three-size explants: 9.0 x 8.0 mm, 6.0 x 5.0 mm, 3.0 x 2.0 mm of endothecium bulb-scale joined by a strip of stem plate and middle-layer bulb-scale joined by a strip of stem plate were cultured on two kinds of the medium: MS +9 mg/L BA +5 mg/ L NAA +0.7% agar +3% cane sugar and MS +5 mg/L BA +9 mg/L NAA +0.7% agar +3% cane sugar, and the culture effects of the three-size explants for Lycoris aurea were studied. RESULTS AND CONCLUSION: The results showed that the...