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A novel electrochemical impedance biosensor based on plasma-polymerized films for detection of biotin

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成果类型:
期刊论文、会议论文
作者:
Deng, Ting*;Cao, Zhong;Shen, Guoli
通讯作者:
Deng, Ting
作者机构:
[Deng, Ting] Cent South Univ Forestry & Technol, Sch Sci, Inst Appl Chem, Changsha 41004, Hunan, Peoples R China.
[Cao, Zhong] Changsha Univ Sci &Technol, Sch Chem & Biol Engn, Hunan Provin Key Lab Mat Protect Elect Power & Tr, Changsha 410004, Hunan, Peoples R China.
[Shen, Guoli; Deng, Ting; Cao, Zhong] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Bio Sensing & Chemomet, Changsha 410082, Hunan, Peoples R China.
通讯机构:
[Deng, Ting] C
Cent South Univ Forestry & Technol, Sch Sci, Inst Appl Chem, Changsha 41004, Hunan, Peoples R China.
语种:
英文
关键词:
Biosensor;Biotin;Electrochemical impedance spectroscopy (EIS);Metastable molecular complex;Plasma-polymerized film (PPF)
期刊:
Advanced Materials Research
ISSN:
1022-6680
年:
2011
卷:
239-242
页码:
1653-1656
会议名称:
International Conference on Chemical Engineering and Advanced Materials
会议论文集名称:
Advanced Materials Research
会议时间:
MAY 28-30, 2011
会议地点:
Changsha, PEOPLES R CHINA
会议主办单位:
[Deng, Ting] Cent South Univ Forestry & Technol, Sch Sci, Inst Appl Chem, Changsha 41004, Hunan, Peoples R China.^[Cao, Zhong] Changsha Univ Sci &Technol, Sch Chem & Biol Engn, Hunan Provin Key Lab Mat Protect Elect Power & Tr, Changsha 410004, Hunan, Peoples R China.^[Deng, Ting;Cao, Zhong;Shen, Guoli] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Bio Sensing & Chemomet, Changsha 410082, Hunan, Peoples R China.
会议赞助商:
Changsha Univ Sci & Technol, James Cook Univ, Hunan Univ, S Central Univ China, Hunan Normal Univ, Xiangtan Univ
主编:
Cao, Z Cao, XQ Sun, L He, YH
出版地:
KREUZSTRASSE 10, 8635 DURNTEN-ZURICH, SWITZERLAND
出版者:
TRANS TECH PUBLICATIONS LTD
ISBN:
978-3-03785-123-4
基金类别:
State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University [200903]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [20775010, 21075011, 21075032, 21005026]; Hunan Provincial Natural Science Foundation of ChinaNatural Science Foundation of Hunan Province [09JJ3016, 10JJ5002]; Foundation of Science and Technology Agency of Hunan Province of China [2009FJ3173, 2010FJ3167]; Foundation of Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation of China [2010CL09, 2011CL06]; Program for New Century Excellent Talents in UniversityProgram for New Century Excellent Talents in University (NCET) [NCET-10-0138]; Ministry of Education of the People's Republic of ChinaMinistry of Education, China
机构署名:
本校为其他机构
院系归属:
化学与生物工程学院
摘要:
An electrochemical impedance biosensor was proposed for the detection of small molecule biotin based on the plasma-polymerized films (PPF) and the bioaffinity difference between an analyte (biotin) and an analogue compound (HABA) in binding avidin. Avidin formed a metastable complex with 2-[(4-hydroxyphenyl)azo]benzoic acid (HABA) immobilized on the electrode surface. When the sensor contacts a sample solution containing biotin, the avidin was released from the sensor surface to form a more stable complex with biotin in solution. The impedance ...

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