期刊论文

Method of Chromosome Flaking for Barleyand ＆ Maize and its Karyotype Analysis

中文

大麦与谷类科学

1673-6486

2008

2

8-11

10.14069/j.cnki.32-1769/s.2008.02.008

湖南省教育厅基金资助（06C089）；

本校为第一机构

运用去壁低渗火焰干燥法制备大麦和玉米染色体片,然后对玉米、大麦的染色体核型进行研究。结果表明:该法使细胞质残留较少和染色体更分散,为细胞学研究提供清晰度高的染色体制片;通过核型分析得到,大麦根尖细胞染色体数目为2n=14,核型公式为2n=2x=14=12m（2SAT）+2 sm（2SAT）,玉米根尖细胞染色体数目为2n=20,其核型公式为2n=20=12m+8 sm（2 eats）。

Chromosome flaking of barley and maize were made by taking off cliff, low pressure seeping ana arying, then the karyotype were analysised. The results showed that little cytoplasm remained, more dispersed of chromosomes and more convenience for cytology research. Chromosomes number of barley was 2n = 14, and maize was 2n =20. The karyotype formula of barley was 2n =2x = 14 = 12m （2SAT） ＋2sm （ 2SAT ） , an...