To obtain the general sample size formula designed for fixed sample size,the mathematical meaning of Fisher's information can be used in hypothesis testing to calculate the variance of Poisson distribution.The formula is also applied to the sample size calculation o...MORE To obtain the general sample size formula designed for fixed sample size,the mathematical meaning of Fisher's information can be used in hypothesis testing to calculate the variance of Poisson distribution.The formula is also applied to the sample size calculation of the two estimates of Poisson distribution,the difference a...