版权说明 操作指南
首页 > 成果 > 详情

Lowering Entropic Barriers in Triplex DNA Switches Facilitating Biomedical Applications at Physiological pH.

认领
导出
Link by DOI
反馈
分享
QQ微信 微博
成果类型:
期刊论文
作者:
Lei, Yanli;Li, Chuangchuang;Ji, Xinyue;Sun, Haiyan;Liu, Xiaowen;...
作者机构:
[Li, Chuangchuang; Lei, Yanli; Sun, Haiyan] Changsha University of Science and Technology, Hunan Provincial Key Laboratory of Cytochemistry, School of Food and Bioengineering, CHINA
[Ji, Xinyue] Changsha University of Science and Technology, Hunan Provincial Key Laboratory of Cytochemistry, School of Chemistry and Chemical Engineering, CHINA
[Liu, Xiaowen; Mao, Zenghui] Changsha Hospital for Maternal & Child Health Care, Hunan Provincial Key Laboratory of Regional Hereditary Birth Defects Prevention and Control, CHINA
[Chen, Weiju] Changsha University of Science and Technology, Hunan Provincial Key Laboratory of Cytochemistry,School of Chemistry and Chemical Engineering, CHINA
[Qing, Zhihe] Changsha University of Science and Technology, Hunan Provincial Key Laboratory of Cytochemistry, School of Chemistry and Food Engineering, 296 Wanjiali South Road, 410114, Changsha, CHINA
语种:
英文
关键词:
Biosensing;Cell capture and release;Entropic barriers;Physiological pH;Triplex DNA switch
期刊:
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
ISSN:
1433-7851
年:
2024
页码:
e202402123
机构署名:
本校为第一机构
摘要:
Triplex DNA switches are attractive allosteric tools for engineering smart nanodevices, but their poor triplex-forming capacity at physiological conditions limited the practical applications. To address this challenge, we proposed a low-entropy barrier design to facilitate triplex formation by introducing a hairpin duplex linker into the triplex motif, and the resulting triplex switch was termed as CTNSds. Compared to the conventional clamp-like triplex switch, CTNSdsincreased the triplex-forming ratio from 30% to 91% at pH 7.4 and stabilized the triple-helix structure in FBS and cell lysate. ...

反馈

验证码:
看不清楚,换一个
确定
取消

成果认领

标题:
用户 作者 通讯作者
请选择
请选择
确定
取消

提示

该栏目需要登录且有访问权限才可以访问

如果您有访问权限,请直接 登录访问

如果您没有访问权限,请联系管理员申请开通

管理员联系邮箱:yun@hnwdkj.com