期刊论文

中文

生物资源

2096-3491

2016

38

4

55-59

10.14188/j.ajsh.2016.04.011

本校为第一机构

以蛋白酶解大豆的完全氨基酸浓度为考察指标,在对提取溶剂及溶剂浓度、提取时间、料液比、提取温度等因素进行工艺优化后,采用正交试验设计进一步确定各因素综合效果,并进行验证试验。结果表明,较佳提取工艺为A2B1C1D3,综合考虑经济、资源利用以及效果等多个因素,确定最佳提取工艺为A1B1C1D1,即：提取时间为2.5h、提取温度为45℃、料液比为1∶45、乙醇浓度为55%。优选得到的提取工艺稳定、可行。

This study used the concentration of complete amino acids from protease hydrolyed soybean as an indicator of the extraction process. Extraction solvent and its concentration, extraction time, solid-liquid ratio, extraction temperature, etc. were optimized and orthogonal experiments were performed to further examine the comprehensive effects of the factors. Results showed that the optimized extraction process was A2B1C1D3. Considering economic, resource utilization and the effects, we determined that the optimal extraction process was A1B1C1D1, ...