期刊论文

Li, D.-Q.

中文

生态学报

1000-0933

2009

29

4

1971-1979

10.3321/j.issn:1000-0933.2009.04.042

基金项目：国家科技部科技基础条件平台建设资助项目（2005DKA21404） 中央公益型科研院所基本科研业务费专项资金资助项目（CAFRIF200713）

本校为第一机构

云南高黎贡山是中国生物多样性保护具有全球意义的关键区域,研究该地区土壤中降解纤维素真菌(cellulose-decomposing fungi,CDF)多样性及分布状况对筛选高效CDF和相关功能基因研究具有重要意义.利用羧甲基纤维素(CMC)刚果红平板和PDA平板,分离培养了高黎贡山海拔960～2900m之间0～20cm深度的土壤样品的好氧CDF,通过液体发酵鉴定其纤维素酶活力,并对其rDNA内转录间隔区(ITS)序列进行分析,用Neighbor-Joining法构建系统进化树.结果发现中低海拔地区土壤CDF数量高于高海拔地区,且数量与样地海拔、土壤含水量、温度、有机质和全氮含量呈显著相关(P

In order to understand the diversity of culturable cellulose-decomposing fungi(CCDF) ,ten surface(0-20cm) soil samples collected at the altitude ranging from 960m to 2900m, in Gaoligongshan Mountain, where are the key areas for biodiversity protection in the world, were studied. Cellulose-decomposing aerobic fungal isolates were obtained using both Carboxymthyl cellulose-congo red (CMC-CR) plates and potato dextrose agar (PDA) plates from the soils. Polysaccharide hydrolase (Endoglucanase) activities of the isolates were determined by liquid fermentation. The plate counting results showed that...