National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31401566, 31601550]; National Key R&D Program of China [2016YFF0203701]
机构署名:
本校为第一且通讯机构
摘要:
In order to avoid the occurrence of false positives and false negatives caused by conventional enzyme-linked immunosorbent assay (ELISA), we established a novel indirect competitive MOF-linked immunosorbent assay (MOFLISA) method for the high throughput and high sensitive detection of aflatoxin B1. This method replaces the natural enzyme with functional MOFs to catalyze a chromogenic system. As a result, the limit of detection (LOD) of the MOFLISA method was 0.009 ng·mL−1 with a linear working range from 0.01 to 20 ng·mL−1. The developed MO...