Single-atom nanozymes (SAzymes) with well-defined metal–nitrogen–carbon coordination structures are of great interest for the development of colorimetric biosensing. However, their catalytic efficiency and specificity is restricted due to the limited number of single metal atoms that can serve as catalytic centre. Therefore, the construction of SAzymes with high activity and specificity is vital but remains challenging. To address these issues, we prepared a bimetallic SAzymes with an independent iron and cobalt structure (FeCo/NC), and the o...