期刊论文

Rapid and Sensitive Detection of L-Cysteine Based on Mono(6-mercapto-6-deoxy) -β-cyclodextrin Modified Gold Electrode

Cao Zhong

中文

高等学校化学学报

0251-0790

2020

41

2

268-276

10.7503/cjcu20190448

Supported by the National Natural Science Foundation of China(Nos. 31527803, 21545010) and the Natural Science Foundation of Hunan Province, China(No. 2017JJ1026).

本校为第一且通讯机构

将单(6-巯基-6-去氧) -β-环糊精(HS-β-CD)通过金硫键自组装在金电极(GE)表面,构建了一种简单、快速、灵敏的超分子识别L-半胱氨酸(L-Cys)的电位型电化学传感器.通过循环伏安法和交流阻抗法研究了膜表面的电化学行为;通过扫描电子显微镜(SEM)和X射线光电子能谱(XPS)表征了电极表面的膜组装效果,其作用机制是固定在金电极表面的HS-β-CD空穴可通过分子间作用力吸附结合带负电的L-Cys,使电极表面的膜电位发生改变,导致对L-Cys的超分子选择性识别作用,从而实现对L-Cys的定量分析.在优化的实验条件下,该电极在pH= 6.0的磷酸盐缓冲溶液中对L-Cys有良好的电位响应性能,线性范围为1.0×10~(-7)~ 1.0×...

A simple, rapid and sensitive L-cysteine(L-Cys)potentiometric electrochemical sensor was constructed by using mono(6-mercapto-6-deoxy)-β-cyclodextrin(HS-β-CD) self-assembled on surface of gold electrode(GE) via gold-sulfur bond. The electrochemical behavior of the electrode membrane surface was investigated through cyclic voltammetry and impedance analysis. Scanning electron microscopy and X-ray photoelectron spectroscopy have also demonstrated the film assembly effectiveness of the electrode. And the interaction mechanism of the electrode wa...