期刊论文

Breeding of temperature-sensitive mutant from Saccharomyces cerevisiae

中文

食品与机械

1003-5788

2016

32

8

9-12,16

10.13652/j.issn.1003-5788.2016.08.003

2013A024:清远市科技计划 2014A023:清远市科技计划 2013FJ4036:湖南省科技计划

本校为第一机构

为获得酵母胞内产物的高效分泌,以酿酒酵母二倍体(2n)及单倍体(n)为出发菌,经紫外诱变处理,筛选获得5株可能具有温度敏感性的突变株。为进一步鉴定,以野生菌Y_1为对照菌,测定其胞外核酸、蛋白质及果糖-1,6-二磷酸(FDP)的含量,发现突变菌株Ts_4(2n)自溶程度最高,其核酸、蛋白质渗透率达1.709,1.483,胞外FDP浓度为38 μg/mL,较Y_1(2n)提高了24.1%。此外突变株Ts_2~*(n)、Ts_3~*(n)也会发生一定程度的自溶。因此,该试验共获得温度敏感性突变株3株,即Ts_4(2n)、Ts_2~*(n)和Ts_3~*(n)。

In order to obtain the efficient secretion of intracellular product in yeast, as the original strain,Saccharomyces cerevisiae (2n), (n) were mutagenized with UV treatment, five mutants strain may have temperature-sensitive were selected. To further identify, Y_1 was used as the control bacterium, and its content of extracellular nucleic acids, proteins and FDP were determined, find that the highest degree of autolysis was Ts_4(2n), it's content of nucleic acid and protein in extracellular environment were 1.709, 1.483, the concentration of FDP ...