期刊论文

Devise and synthesis of fluorescent probe for cyanide detection in food based on a peptide receptor

中文

食品与机械

1003-5788

2016

32

10

44-47,62

10.13652/j.issn.1003-5788.2016.10.010

国家自然科学基金项目（编号：U1404215,31171627,31071523） 湖南省食品药品监督管理局食品药品安全科技项目（编号：湘食药科R201516）

本校为其他机构

以7-二乙胺基香豆素-3-羧酸、甘氨酸、组氨酸为原料,采用标准多肽合成法成功合成了水溶性的荧光探针分子香豆素-甘氨酸-甘氨酸-组氨酸(Coumarin-Gly-Gly-His,C-GGH),利用GGH三肽对Cu~(2+)的络合作用形成C-GGH-Cu~(2+)络合物,使探针分子C-GGH的荧光猝灭;然后,通过向体系中加入氰化物后,CN~-对Cu~(2+)的强络合作用形成稳定的络合物[Cu(CN)_x]而使探针分子荧光逐渐恢复,以此实现对氰化物的定量检测。该法检测限为0.015 μmol/L,远低于国家标准(GB 8537-2008)中要求最严的矿泉水中氰化物的限量值0.01 mg/L(相当于摩尔浓度0.385 μmol/L),该探针分子对CN~-的检测不受SCN~-、F~-、Cl~-、Br~-、I~-、PO...

Cyanide is considered to be one of the most toxic species in food while the existing detection methods involve complex operation and will produce toxicity to inspectors. In this study, a water-soluble fluorescent probe(C-GGH) was synthesized by HOBT/DIC protocol in solution using 7-diethylaminocoumarin-3-carboxylic acid, Glycine and Histidine. Due to the presence of the recognition unit GGH, the probe C-GGH can conjugated with Cu~(2+) to formed non-fluorescent C-GGH-Cu~(2+) complex. Due to the strong binding affinity of CN~- to Cu~(2+), CN~- ca...