期刊论文

Enzyme-linked sensitization biosensor detection of bisphenol A in food matrix Based on magnetic nanomaterials

中文

食品与机械

1003-5788

2017

33

10

47-51

10.13652/j.issn.1003-5788.2017.10.001

31401566:国家自然科学基金 31601550:国家自然科学基金 2016YFF0203701:国家重点研发计划

本校为第一机构

以纳米材料为载体,以双酚A适配体及互补链为生物识别单元,建立了基于功能化磁纳米粒子及金纳米粒子的辣根过氧化物酶酶联增敏检测平台,可实现对食品基质中双酚A的快速前处理和痕量检测,该方法将可与双酚A特异性结合的适配体偶联于磁性纳米材料上,再与适配体互补链及辣根过氧化物酶(HRP)修饰的金纳米粒子进行竞争性结合,通过HRP对底物的催化水解反应引起450 nm处特征峰的变化来定量检测双酚A。结果表明:该检测体系在0~100 ng/mL时具有线性关系(R~2=0.978 4),检测限低至0.5 pg/mL,具有较好的实用性,为更好地检测食品基质中的双酚A提供了有力的技术支持。

A horseradish peroxidase enzyme-sensitized detection plat-form based on functionalized magnetic nanoparticles and gold nanop-articles was established with the nanomaterials as the carrier and the bisphenol A aptamer and the complementary strand as the biometric recognition unit. The method about rapid pretreatment and trace detection of bisphenol A in food matrix was established. The BPA aptamer binding to the magnetic nanomaterials could compete with the complementary strands of the aptamer and the horn root peroxi- dase （HRP） modified gold ...