期刊论文

Highly Sensitive Detection of Bisphenol A Based on FRET from Up-conversion Nanoparticles to Gold Nanoparticles

中文

食品与机械

1003-5788

2018

34

9

83-87

10.13652/j.issn.1003-5788.2018.09.017

国家自然科学基金（编号：31401566） 国家重点研发计划（编号：2016YFF0203701） 粮食深加工与品质控制湖南省2011协同创新项目 常熟市科技发展计划（社会发展类）项目（编号：CS201605） 苏州市科技计划项目（编号：SNG201617）

本校为第一机构

制备了粒径为13nm的金纳米粒子,在其表面修饰双酚A（BPA）适配体作为能量受体探针;并利用聚丙烯酸（PAA）包覆油溶性的上转换荧光纳米材料（UCNPs）制备水溶性的UCNPs,在其表面修饰适配体互补链形成功能化UCNPs作为能量供体,构建了基于FRET原理的BPA生物传感检测平台。结果表明:该检测体系在1×10-9～1×10-3 mol/L时具有良好的线性关系（R2=0.992 3）,检出限低至1×10-10 mol/L。

The 13 nm gold nanoparticles were synthesized and were modified with Bisphenol A(BPA)aptamer.The oil-solubility Upconversion nanoparticles(UCNPs)were modified with polyacrylic acid(PAA)to form water-solubility UCNPs.Then water-solubility UCNPs were coated with the complementary DNA strand.Based these nanoparticles,a FRET aptasensor for BPA was successfully fabricated by using Up-conversion nanoparticles as energy donor and gold nanoparticles as energy acceptor.The results show that the detection system has a good linear relationship at 1×10~(-...