期刊论文

中文

食品与机械

1003-5788

2018

34

3

151-155

10.13652/j.issn.1003-5788.2018.03.032

湖南省自然科学基金项目（编号:2018JJ2423）；

本校为第一机构

为进一步提高经过DA201-C型大孔吸附树脂脱盐、 40%乙醇梯度洗脱的大米免疫活性肽RPHs-A3组分的纯度和免疫活性,采用中压离子交换色谱对其进行进一步分离纯化,并对分离得到组分的免疫活性进行了评价。结果表明,采用WorkBeads 40Q强阴离子交换树脂、2.6cm×40cm色谱柱对RPHs-A3组分进行分离的最佳条件为:上样浓度30mg/mL,上样量5 mL,起始缓冲液A为pH 9.0的0.01mol/L Tris-HCl,洗脱缓冲液B为含1mol/L NaCl的pH 9.0、0.01 mol/L Tris-HCl,上样、洗脱流速分别为1, 10mL/min。共收集到5个组分,其中RPHs-A3-B3组分在MTT试验和小鼠巨噬细胞脂多糖(LPS)炎症模型中皆表现出较高免疫活性,对RAW264.7细胞具...

Medium-pressure ion exchange chromatography was used for further fractionation of rice immunoactive peptides obtained from Trypsin hydrolyzation and gradient ethanol elution with DA201-C macroporous adsorption resin（40% ethanol fraction）.The results showed that the optimal parameters for fractionation of RPHs-A3 fractions by WorkBeads 40 Qon a 2.6 cm×40 cm columns were as follows：Sample：5 mL,30 mg/mL 40% ethanol fraction;Equilibration buffer A：pH 9.0,0.01 mol/L Tris-HCl;Elution Buffer B：pH 9.0,0.01 mol/L Tris-HCl containing 1 mol/L NaCl;...