期刊论文

Cheng, Y.-H.

中文

现代食品科技

1673-9078

2016

32

1

13-21

10.13982/j.mfst.1673-9078.2016.1.003

31171627:国家自然科学基金 31071523:国家自然科学基金 2012BAD34B02:国家科技支撑计划

本校为第一且通讯机构

本文研究了麦胚肽(RVF)对人神经瘤细胞SH-SY5Y损伤的保护作用.采用体外细胞培养法,以人神经瘤细胞SH-SY5Y为材料,建立了饥饿损伤和H2O2损伤模型.结果表明:在40个不同浓度与作用时间的组别中,对SH-SY5Y细胞具有显著营养功能的只有无血清饥饿模型中200 μmol/L RVF作用12 h与含1％血清饥饿模型中500 μmol/L RVF作用24 h两个组别,且不具有剂量与时间依赖性.在H2O2诱导的细胞氧化损伤模型中,200 μmol/L的RVF将细胞存活率分别提高了25.05％(MTT法)和21.90％(SRB法);200μmol/L RVF预孵育时间延长至12 h时,RVF极显著地提高了细胞存活率,且具有剂量与时间依赖性;细胞形态检测结果表明RVF干预后,SH-SY...

The protective effects of wheat germ peptide (RVF) on the SH-SY5Y human neuroblastoma cell line were investigated in this study. Starvation- and hydrogen peroxide (H2O2)-induced injury models were established by in vitro cell culturing of an SH-SY5Y human neuroblastoma cell line. The SH-SY5Y cells were treated with 40 RVFs at different concentrations and incubation times. The results showed significant nutritional functions only in the group pre-incubated with 200 μmol/L RVF for 12 h in a no-serum starvation-induced injury model and the group ...