期刊论文

Zhan, X.-H.(zhanxueh@163.com)

中文

中南大学学报(自然科学版)

1672-7207

2009

40

3

587-591

基金项目：国家自然科学基金资助项目（50774016）：教育部科学技术研究重点资助项目（208095） 湖南省教育厅重点科研基金资助项目（07A006）

本校为第一且通讯机构

设计一种基于荧光增强原理检测伯胺的新方法.利用邻苯二甲醛与烯丙基硫醇、乙二醇二甲基丙烯酸酯(EDMA)在偶氮二异丁腈(AIBN)引发下热聚合制得了一种新型聚合物颗粒.该聚合物颗粒本身的荧光很弱,但可与伯胺作用,形成具有强荧光的异吲哚类化合物.利用聚合物颗粒的这一性质测定氨基乙酸.实验结果表明:在pH值为8.0的磷酸盐缓冲溶液/乙醇(体积比4:1)混合介质中,控制聚合物颗粒质量浓度为1.5 g/L,聚合物颗粒与氨基乙酸反应3 h以上,反应可进行完全,混合悬浮液的荧光强度可达到稳定值.聚合物颗粒悬浮液的相对荧光强度对氨基乙酸的浓度为5.0×10~(-6)～7.O×10~(-5) mol/L时有线性响应,线性相关系数为0....

A new method based on fluorescence enhancement was designed for determination of primary amine. The polymer particles were prepared by copolymerization of phthalic aldehyde, allyl mercaptan and ethylene glycol dimethacrylate (EDMA) after heating. The fluorescence intensity of the polymer particles was very weak. A compound, which had isoindole unit in its molecule with strong fluorescence intensity, would form when the polymer particles were reacted with primary amine. This property of the polymer particles was used to assay aminoacetic acid. The experimental results show that the fluorescence...