期刊论文

Zhan, X.-H.(zhanxueh@163.com)

中文

中南大学学报(自然科学版)

1672-7207

2010

41

3

890-895

20976017:国家自然科学基金 50774016:国家自然科学基金

本校为第一且通讯机构

以一种纯天然产物对羟基桂皮醇(4-hydroxycinnamic alcohol, HCA)为辣根过氧化物酶(HRP)底物, 建立对羟基桂皮醇-辣根过氧化物酶-过氧化氢新体系. 对羟基桂皮醇本身只有极弱的荧光, 在HRP催化下可被H_2O_2氧化成二聚体产物, 该二聚体在315 nm的激发光下能发射波长为467 nm的强荧光, 并且反应体系荧光强度增加值与HRP量在一定浓度范围内呈线性相关. 根据此关系和竞争型免疫定量原理, 建立兔布氏杆菌抗体测定的荧光酶联免疫传感体系, 并对免疫测定条件如pH值、HRP-BrAb用量、BSA和流速等进行优化. 运用制备传感体系测定兔布氏杆菌抗体的质量浓度线性范围为2.7~90μg/L, 检测限为2.7μg/L, 相对标准...

A natural product, 4-hydroxycinnamic alcohol (HCA) was used as a substrate for HRP in enzyme-linked fluoroimmunoassay. In enzymatic reaction procedure, HRP-Brucella melitensis antibody conjugate (HRP-BrAb) catalyze the polymerization of HCA by H2O2, and the HCA is partly converted to polymers, a fluorescent species. The fluorescence increase of the HRP-enzymatic product at emission of 467 nm (excitation at 315 nm) is proportional to the concentration of HRP-BrAb binding to the Brucella melitensis antigens, which were entrapped in cellulose-para...