期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2009

35

4

348-351

基金项目：教育部科学技术研究重点项目（204101）

本校为其他机构

通过农杆菌介导,将几丁质酶基因和葡聚糖酶基因导入籼稻E32、9311、特青、盐恢559等4个栽培品种的愈伤组织中,经筛选与培养获得转基因植株.分子检测证明外源基因已整合到受体植株基因组中.抗病性鉴定的结果表明,转基因植株抗稻瘟病的能力有明显提高.

Chitinase and β-1,3 glucanase were introduced into the catli of indica rice cultivars E32, 9311, Teqing and Yanhui559 mediated by Agrobacterium. Transgenic plants were obtained. PCR and dot blot revealed that foreign genes were integrated into the genome of these plants. At the same time, the field bioassay revealed that some transgenic plants were more res...