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Targeted gene therapy for intracranial aneurysm using SOX17-CRISPRa

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成果类型:
期刊论文
作者:
Dujie Xie;Yi Yuan;Panyao Long;Juan Huang;Mi Jiang;...
通讯作者:
Shuntong Hu
作者机构:
[Yi Yuan; Panyao Long; Juan Huang; Yiwei Chen; Xiaobo Li; Zhi Song; Shuntong Hu] Department of Neurology, The Third Xiangya Hospital, Central South University, Changsha 410013, China
Department of Neurology, University of Michigan, Ann Arbor, MI 48109, USA
Department of Anesthesiology, The Third Xiangya Hospital, Central South University, Changsha 410013, China
Clinical Research Center, The Third Xiangya Hospital, Central South University, Changsha 410013, China
College of Automotive and Mechanical Engineering, Changsha University of Science and Technology, Changsha 410114, China
通讯机构:
[Shuntong Hu] D
Department of Neurology, The Third Xiangya Hospital, Central South University, Changsha 410013, China
语种:
英文
期刊:
Chemical Engineering Journal
ISSN:
1385-8947
年:
2025
页码:
166492
基金类别:
This study was supported by the Natural Science Foundation of Hunan Province ( 2023JJ40891 ), STI2030-Major Projects ( 2021ZD0200200 ), the National Natural Science Foundation of China ( 82101529 ), the Wisdom Accumulation and Talent Cultivation Project of the Third Xiangya Hospital of Central South University ( YX202213 ).
机构署名:
本校为其他机构
院系归属:
汽车与机械工程学院
摘要:
The aim of this study was to investigate the molecular basis and therapeutic potential of SOX17 activation using a CRISPRa-based gene editing system in treating intracranial aneurysm (IA), supported by targeted delivery through nanomicrospheres. An IA model in mice was established through basal cistern elastase injection and angiotensin II-induced hypertension. RNA sequencing of intracranial arteries identified 77 DEGs significantly enriched in vascular remodeling pathways, particularly Wnt and Hippo signaling. Computational analyses (LASSO and random forest) identified SOX17 as a central regu...

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