期刊论文

Fu-Chun, Gong

中文

分析化学

0253-3820

2007

35

12

1783-1786

10.1016/S1872-2040(08)60009-2

Received 29 March 2007; accepted 22 July 2007 * Corresponding author. Email: gfc139@yahoo.com.cn; Tel: +86 731-2618234; Fax: +86 731-2618234 This work was supported by the Natural Science Foundation of Hunan Province of China (No. 07JJ3020) and the National Natural Science Foundation of China (No. 20775010). Copyright © 2007, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences. Published by Elsevier Limited. All rights reserved.

本校为第一且通讯机构

一种纯天然产物白藜芦醇用作辣根过氧化物酶（HRP）底物。对其化学性质的研究证实，白藜芦醇在空气中较稳定，对HRP、H_2O_2的电化学响应性能优于传统HRP底物，对人体无毒害。白藜芦醇在HRP催化下可被H_2O_2氧化成醌，产物醌在电极上于一376mV处可被还原，其电流的大小与HRP的浓试在一定浓度范围内呈线性相关。将兔布氏杆菌抗原包埋在石墨．石蜡基质中制备了测定兔布氏杆菌抗体的电化学酶联免疫传感器，该传感器测定兔布氏杆菌抗体的线性范围为3×10~(-4)～1．65×10~(-2)g／L；检出限为1×10~(-4)g／L；RSD为4．6％。本方法制备免疫传感器的电化学性能稳定，抗原活性保持良好。

As a natural product, resveratrol was evaluated as a potential substrate for horseradish peroxidase (HRP) and was applied to the amperometric enzyme-linked immunosensing assays. The sensors based on HRP-IgG were constructed by dispersing graphite, BrAg, and paraffin wax at room temperature. Optimal measurements of resveratrol substrate for HRP were investigated. The results of the comparison studies indicate that resveratrol is more feasible for HRP and more stable in the air than o-phenylenediamine, o-benzophenone aniline, and 3,3′, 5,5′-tet...