期刊论文

Wang Xiao-Yong

中文

分析化学

0253-3820

2010

38

10

1457-1461

10.3724/SP.J.1096.2010.01457

2009FJ4061:湖南省科技计划 2009CK3069:湖南省科技计划

本校为其他机构

合成了荧光标记物长春西汀酸和示踪物磺胺二甲氧基嘧啶-长春胺酸,并采用薄层色谱法对示踪物进行提纯。以磺胺二甲氧基嘧啶为分析对象,利用示踪物与磺胺二甲氧基嘧啶抗体的特异性反应,采用荧光偏振免疫分析方法（FPIA）研究了荧光标记物的荧光光谱特性。此荧光标记物在357nm的激发光下,于453nm处产生一个荧光发射峰;以荧光素为对照,考察了本方法的灵敏性。结果显示,荧光素的检出限仅为2.7μg/L,而本方法的检测范围为0.5～146μg/L,检出限达到0.5μg/L。本方法的RSD为3.0%,重现性好;其它磺胺类药物对磺胺二甲氧基嘧啶的测定不产生明显干扰。运用本方法检测了饲料中磺胺二甲氧基嘧啶,结果与HPLC法相...

The fluorescence labeling apovincaminic acid (AVA) and tracers apovincaminic acid-sulfadimethoxine were synthesized and used in the fluorescence polarization immunoassay method (FPIA) for the analysis of sulfadimethoxine (SMX). By using AVA as indicating materials, tracers apovincaminic acid-sulfadimethoxine (AVA-SMX) were prepared, which were purified by thin layer chromatography and identified by FPIA. The fluorescence properties of AVA and the characteristic performance of tracers immunoreacting with SMX antibodies were investigated by using tracers 5-aminofluorescein-sulfadimethoxine conju...