A systemic experimental investigation involving scanning electron microscopy, Vickers microhardness tester, X-ray diffraction, three-axis piezoelectric dynamometer, and thermocouple was carried out to analyze the affected layers formed in grinding of AISI 52100 steel. The formation mechanisms and properties of affected layer at different grinding conditions were investigated. It is found that the phase transformation, as well as retained austenite and white layer, can be formed at the grinding temperature below the nominal phase transformation ...