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Characterization and identification of a fraction from silver carp (Hypophthalmichthys molitrix) muscle hydrolysates with cryoprotective effects on yeast

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成果类型:
期刊论文
作者:
Wang, Faxiang;Cui, Meili;Liu, Haidong;Li, Xianghong*;Yu, Jian;...
通讯作者:
Li, Xianghong;Liu, Yongle
作者机构:
[Wang, Faxiang; Yu, Jian; Liu, Yongle; Li, XH; Liu, YL; Cui, Meili; Li, Xianghong; Liu, Haidong; Huang, Yiqun] Changsha Univ Sci & Technol, Sch Chem & Food Engn, Hunan Prov Engn Technol Res Ctr Aquat Food Resour, Changsha 410114, Peoples R China.
通讯机构:
[Li, XH; Liu, YL] C
Changsha Univ Sci & Technol, Sch Chem & Food Engn, Hunan Prov Engn Technol Res Ctr Aquat Food Resour, Changsha 410114, Peoples R China.
语种:
英文
关键词:
Freshwater fish;HPLC-MS/MS;Parvalbumin;Antifreeze peptide;Amino acid sequence
期刊:
LWT
ISSN:
0023-6438
年:
2021
卷:
137
页码:
110388
基金类别:
CRediT authorship contribution statement Faxiang Wang: Methodology, Investigation, Data curation, Writing - original draft. Meili Cui: Investigation, Data curation, Formal analysis. Haidong Liu: Investigation, Validation. Xianghong Li: Conceptualization, Project administration. Jian Yu: Resources. Yiqun Huang: Writing - review & editing. Yongle Liu: Supervision, acquisition.
机构署名:
本校为第一且通讯机构
院系归属:
化学与生物工程学院
摘要:
This study was aimed to characterize and identify a fraction from silver carp muscle hydrolysates (SCMHs) with cryoprotective effects on yeast. The SCMHs were obtained from the hydrolysis of silver carp (Hypophthalmichthys molitrix) muscle with Protamex at 50 degrees C for 15-240 min. The addition of SCMH generated from 30 min of proteolysis (SCMH-30) led to an increase in the yeast survival rate from 0 - 1% (control) to 82-91% after 1-2 freeze-thaw cycles. The SCMH-30 was further separated into two fractions using gel filtration chromatography. The fraction F2 with lower average molecular wei...

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