In this study, a chalcone-based fluorescent probe (2-HPC) was synthetized and used to detect N 2 H 4 . Upon addition of N 2 H 4 , the fluorescence of 2-HPC significantly decreases at 478 and increases at 445 nm. The sensing mechanism was investigated using nuclear magnetic resonance (NMR) and mass spectrometry (MS). This fluorescence enhancement-based method offers a low detection limit of 12.3 nM. Additionally, a simple 2-HPC-loaded silica gel sheet device was also developed and employed for monitoring N 2 H 4 in water samples. Importantly, ...