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An electrochemical impedimetric sensing platform based on a peptide aptamer identified by high-throughput molecular docking for sensitive l-arginine detection

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成果类型:
期刊论文
作者:
He, Yumin;Zhou, Li;Deng, Lei;Feng, Zemeng*;Cao, Zhong;...
通讯作者:
Feng, Zemeng
作者机构:
[He, Yumin; Yin, Yulong; Feng, Zemeng] Chinese Acad Sci, Hunan Prov Key Lab Anim Nutr Physiol & Metab Proc, Hunan Prov Engn Res Ctr Hlth Livestock & Poultry, Inst Subtrop Agr,Minist Agr,Key Lab Agroecol Proc, Changsha 410125, Hunan, Peoples R China.
[Zhou, Li; Cao, Zhong] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Collaborat Innovat Ctr Micro Nano Biosensing & Fo, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Changsha 410004, Hunan, Peoples R China.
[He, Yumin; Yin, Yulong] Hunan Normal Univ, Coll Life Sci, Anim Nutr & Human Hlth Lab, Changsha 410081, Hunan, Peoples R China.
[Deng, Lei] Cent South Univ, Sch Comp Sci & Engn, Changsha 410075, Peoples R China.
通讯机构:
[Feng, Zemeng] C
Chinese Acad Sci, Hunan Prov Key Lab Anim Nutr Physiol & Metab Proc, Hunan Prov Engn Res Ctr Hlth Livestock & Poultry, Inst Subtrop Agr,Minist Agr,Key Lab Agroecol Proc, Changsha 410125, Hunan, Peoples R China.
语种:
英文
关键词:
Peptide aptamer;Docking;L-Arg;Biosensor;EIS;Isothermal titration calorimetry
期刊:
Bioelectrochemistry
ISSN:
1567-5394
年:
2021
卷:
137
页码:
107634
基金类别:
This work was supported by National Key Scientific Instrument and Equipment Development Projects of China (free application) ( 31527803 ).
机构署名:
本校为其他机构
院系归属:
化学与生物工程学院
摘要:
As a primary building block for protein synthesis, L-arginine (L-Arg) is also a precursor for the synthesis of important metabolites, and is involved in various physiological and pathophysiological processes. L-Arg is a potential biomarker in clinical diagnosis and nutritional status assessment, making it valuable to quantify and monitor this biomolecule. In this study, peptide aptamers that specifically interact with L-Arg were identified by high-throughput molecular docking, and the binding capacities between the synthesized peptide aptamers and L-Arg were then measured by isothermal titrati...

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