期刊论文

中文

食品科学

1002-6630

2009

30

24

38-41

10.3321/j.issn:1002-6630.2009.24.005

(2008AA100801):国家高技术研究发展计划项目 (2008FJ1004):湖南省科技重大专项

本校为第一机构

以早籼米为原料,用还原剂配合碱溶酸沉的方法提取大米蛋白.通过单因素试验与正交试验得出优化的提取条件为碱液浓度0.05mol/L、Na_2SO_3用量为米粉的0.5%、DTT用量为米粉的2.5%、固液比1:12(g/ml)、室温提取4h,在此条件下,大米蛋白提取率可达95.26%.

In this study, a combinatorial method of reductant-alkali solution and acid precipitation was used to extract protein from rice endosperm. The optimal condition for rice protein extraction was determined through single factor and orthogonal experiments to be 0.05 mol/L sodium hydroxide, 0.50% sodium sulfite, 2.50% dithiothreitol, 1：12 （g/ml） of solid-liquid ratio, and 4 h of extraction time. Extraction rate of rice p...