期刊论文

Isolation and Identification of Starch Granule-Associated Proteins (SGAPs) from Indica Rice

中文

食品科学

1002-6630

2018

39

20

240-245

10.7506/spkx1002-6630-201820035

国家自然科学基金面上项目（31771899）；

本校为第一机构

采用十二烷基硫酸钠(sodium dodecyl sulfate,SDS)缓冲体系分离提取碱洗籼米粉中的淀粉粒结合蛋白(starch granule-associated proteins,SGAPs),经高效液相色谱-串联质谱法鉴定表明:碱洗得到上、中、下层籼米淀粉中的SGAPs均主要为淀粉合成酶I(granule-bound starch synthase I,GBSS I),其他组分略有差别;其中以中层SGAPs含量最高,GBSS I达99.8%,谷蛋白质量分数仅为0.11%。采用响应面优化中层淀粉SGAPs的提取参数,得到最优条件为SDS添加量10.90%、煮沸时间4.10 min、MgSO_4浓度0.90 mmol/L,此时SGAPs提取量为(1 028.61±0.05)μg/g。

Starch granule-associated proteins (SGAPs) from indica rice were isolated by sodium dodecyl sulfate (SDS) buffer from alkali washed rice flour and then identified by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Results indicated that the SGAPs from the top, middle and bottom starch layers mainly consisted of granule-bound starch synthase I (GBSS I), which slightly varied with respect to other components. Among these layers, the content of SGAPs in the middle layer was the highest, with GBSS I accounting for up t...