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Highly Sensitive Detection of Bladder Cancer-Related miRNA in Urine Using Time-Gated Luminescent Biochip

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成果类型:
期刊论文
作者:
Wang, Yingqian;Li, Zhihao;Lin, Qiaosong;Wei, Yurong;Wang, Jie;...
通讯作者:
Yuan, Quan
作者机构:
[Wang, Yingqian; Yuan, Quan; Li, Zhihao; Wei, Yurong; Lin, Qiaosong; Li, Yingxue; Wang, Jie] Wuhan Univ, Coll Chem, Minist Educ, Key Lab Analyt Chem Biol & Med, Wuhan 430072, Hubei, Peoples R China.
[Yuan, Quan] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Mol Sci & Biomed Lab, Changsha 410082, Hunan, Peoples R China.
[Yang, Ronghua] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.
通讯机构:
[Yuan, Quan] W
[Yuan, Quan] H
Wuhan Univ, Coll Chem, Minist Educ, Key Lab Analyt Chem Biol & Med, Wuhan 430072, Hubei, Peoples R China.
Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Mol Sci & Biomed Lab, Changsha 410082, Hunan, Peoples R China.
语种:
英文
关键词:
long-lifetime luminescence;photonic crystals;miRNA detection;urine;autofluorescence
期刊:
ACS SENSORS
ISSN:
2379-3694
年:
2019
卷:
4
期:
8
页码:
2124-2130
基金类别:
This work was supported by the National Key R&D Program of China (2017YFA0208000, 2016YFF0100800), National Natural Science Foundation of China (21675120), National Postdoctoral Program for Innovative Talents (BX20180223), National Basic Research Program of China (973 Program, Grants 2015CB932600), Ten Thousand Talents Program for Young Talents. Q. Yuan thanks the large-scale instrument and equipment sharing foundation of Wuhan University.
机构署名:
本校为其他机构
院系归属:
化学与生物工程学院
摘要:
Detection of biomarkers in complex samples is a significant health plan strategy for medical diagnosis, therapy monitoring, and health management. However, high background noise resulting from impurities and other analytes in complex samples has hampered the improvement of detection sensitivity and accuracy. Herein, an ultralow background biochip based on time-gated luminescent probes supported by photonic crystals (PCs) was successfully developed for detection of bladder cancer (BC)-related miRNA biomarkers with high sensitivity and specificity in urine samples. Coupled with the time-gated lu...

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