期刊论文

Cao, Zhong

英文

ANALYTICAL METHODS

1759-9660

2019

11

32

4165-4172

10.1039/c9ay01214a

This work was financially supported by the projects of the National Natural Science Foundation of China (No. 31527803, 21545010 and 21645009) and the Natural Science Foundation of Hunan Province, China (No. 2017JJ1026). Moreover, the support received from the Sino-Danish Center for Education and Research is fully acknowledged.

本校为第一且通讯机构

Herein, DNA-templated copper nanoclusters (CuNCs) synthesized by terminal deoxynucleotidyl transferase (TdT) isothermal nucleic acid amplification (INAA) were proposed for Hg2+ assay. TdT recognized the 3′-OH end of primers and subsequently prolonged the single strand poly-T at 3′-OH with the assistance of deoxythymidine triphosphate (dTTP). The generated products coordinated with the copper ions to form DNA-templated CuNCs, resulting in remarkable fluorescence. The introduction of Hg2+ made the primers form a T-Hg2+-T mismatch duplex, which ...