期刊论文

中文

中国水稻科学

1001-7216

2012

26

5

529-536

10.3969/j.issn.1001-7216.2012.05.003

中国博士后基金面上重点项目（20110490147） 湖南省科技厅项目（2011RS4007） 湖南省教育厅项目（07C069）

本校为第一机构

为在孢子发育时期从蛋白质水平更好地理解细胞质雄性不育的分子机理,对红莲型细胞质雄性不育水稻的不育系粤泰A、保持系粤泰B及其F_1(红莲优6号)单核期花粉总蛋白质采用固相pH梯度等电聚焦/SDS-PAGE(3~10非线性胶条)进行双向电泳分离,利用基质辅助激光解析电离飞行时间质谱(MALDI-TOF/MS)或者LC-MS/MS分析对其中15个差异点进行鉴定,并对已鉴定的蛋白质点进行亚细胞定位和功能分析。发现与可育系相比,不育系有部分参与物质和能量代谢、细胞周期、转录、物质转运等的蛋白质缺失或表达量降低。这些蛋白质包括K~+/H~+协同运输蛋白、锌指蛋白和WD重复蛋白等。这些蛋白质的表达下调或缺失可能与线粒体...

To better understand the molecular mechanism of cytoplasmic male sterility （CMS） at the protein level during the development of microspores, the protein expression profile was analyzed in pollens of the CMS rice line Yuetai A, its fertile maintainer line Yuetai B and their hybrid F1 （Honglianyou 6）. The proteins were separated by two dimensional electrophesis with immobilized pH （3-10 non-linear, NL） gradients as the first dimension and SDS-PAGE as the second, and 15 differentially expressed proteins were identified by Matrix assisted las...