National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21645009, 31527803, 21545010]; Natural Science Foundation of Hunan ProvinceNatural Science Foundation of Hunan Province [2018JJ2419]
机构署名:
本校为第一且通讯机构
院系归属:
化学与生物工程学院
摘要:
Sensitive and specific detection of microRNAs (miRNAs) is of great significance for early cancer diagnosis. Here we report a simple and sensitive fluorescence signal amplification strategy that based on DSN/TdT recycling digestion for miRNA detection. DSN initiates DNA digestion on 3′-phosphate-primer/miRNA heteroduplex which causes miRNA recycle. The digested DNA strands with 3′-OH ends enable TdT to synthesize a polydeoxyguanylic tails on the 3′-end. The DNAs with polydeoxyguanylic tails are converted to double-stranded-DNA prior to initia...