DNA fingerprints of Gastrodia elata Blume were determined using randomly am-plified polymorphic DNA (RAPD) technique, from which the desired DNA segments were selected. Positive DNA clones were obtained by recovering,cloning and identifying of DNA. Novelty of the cloned DNA and its bio-informations were confirmed by sequencing DNA and bioinformatics analyzing. Distribution of the DNA sequence in populations of Gastrodia elata Blume were analyzed using PCR technique. The results showed the DNA sequence is newly discovered, contains plenty of b...