A systematic study on the instability of whistler mode chorus in space plasma by a Kappa distribution was presented. When the anisotropy or the number density of energetic electrons increases, the wave growth rate increases and the frequency range of positive growth rate is broader. If the temperature of energetic electrons is very low or very high, the interaction between the particles and wave are relatively weak and the growth rate is relatively low. The path-integrated gain of whistler-mode chorus was calculated based on ray trace results and it is found that the lower band chorus has a mu...