期刊论文

中文

热带作物学报

1000-2561

2010

31

2

229-234

10.3969/j.issn.1000-2561.2010.02.013

No.2007FJ4139:湖南省科技厅项目 04NK3007:湖南省科技厅项目 08C084:教育厅项目

本校为第一机构

以野生药用植物黄花石蒜(Lycoris aurea Herb.)为材料,选用其鳞茎的内层鳞片、中层鳞片2个不同部位的外植体,研究MS培养基中9种不同BA与NAA的浓度配伍对黄花石蒜鳞片的组织培养效果.结果表明:MS+10 mg/L BA+5 mg/L NAA+0.7%琼脂+3%蔗糖,有利于内层鳞片和中层鳞片外植体不定芽、不定根的发生,产生褐变量也较少,其培养效果显著较好.在培养基中BA、NAA的浓度分别高于或分别低于10、5 mg/L,其培养效果均显著较差.相关分析表明,2种外植体相同BA与NAA浓度配伍的培养效果为极显著正相关,这进一步说明,对一种外植体培养效果较好的BA与NAA的浓度配伍对另一种外植体的培养效果仍然较好.多重比较与相关分析...

Endothecium bulb-scales and mid-layer bulb-scales of bulbs of wild medicinal plant Lycoris aurea Herb.were used as explants for tissue culture on MS medium containing 9 mixtures of 6-benzylaminopurine（BA）and 1-naphthaleneacetic acid（NAA）at different concentrations.The results showed that the explants produced more adventitious shoots and roots with less browning on the medium MS＋10 mg/l BA ＋5 mg/l NAA＋0.7% agar＋3% sucrose but gave evidently poorer cultural results on the medium supplemented with BA and NAA each at the concentration of h...