This study aimed to investigate the improvement effects of a silver carp muscle hydrolysate (SCMH) on freeze-thaw (FT) tolerance of baker's yeast and the related mechanism. Yeast cells with or without addition of SCMH were subjected to 0 to 6 FT cycles, and their cell viability, leavening activity, growth kinetics, morphology, and transcriptome were examined. The results showed that the SCMH-added yeast had significantly higher cell viability (40.7%), leavening activity (473.2 mL·kg -1 ·h -1 ), and maximum specific growth rate (2.63 h -1 ) th...