期刊论文

中文

西北植物学报

1000-4025

2010

30

8

1695-1700

2007FJ4139,04NK3007:湖南省科技厅项目 08C084:教育厅项目

本校为第一机构

以药用植物黄花石蒜为材料,选用其不同部位的8种外植体,在2种不同BA/NAA浓度比的MS+0.7%琼脂+3%蔗糖基本培养基上培养,以明确黄花石蒜不同部位外植体的组织培养效果. 结果显示:带底盘双内层鳞片外植体在BA/NAA为10/5的培养基上不定芽的发生率为90.33%、不定根为87.00%、根芽俱有为85.00%、褐变量为6.33%,在BA/NAA为5/10的培养基上不定芽的发生率为46.67%、不定根为82.33%、根芽俱有为35.33%、褐变量为16.67%,与其他外植体均差异显著. 8种外植体在2种培养基上的培养效果均极显著正相关. 研究表明,用黄花石蒜最佳外植体为带底盘双内层鳞片,其次为带底盘双中层鳞片,并用MS+10 mg. L~(-1)BA+5mg. L~...

Using wild medicinal plant Lycoris aurea Herb.as the experimental materials,eight kinds of explants from L.aurea Herb.were cultured on two kinds of culture medium：MS＋10 mg·L^-1 BA＋5 mg·L^-1 NAA＋0.7% agar＋3% cane sugar,and MS＋5 mg·L^-1 BA＋10 mg·L^-1 NAA＋0.7% agar＋3% cane sugar.The effects on tissue culture of the eight kinds of explants were studied.The results showed that the adventitious bud rate,adventitious root rate,adventitious bud and root rate,and browning rate of the double endothecium＇s bulb-scale explants cultured on the...