Peptide aptamer-based polyaniline-modified amperometric biosensor for L-lysine detection in real serum samples

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成果类型：

期刊论文

作者：

Wang, Wenjing;He, Yumin;Deng, Lei;Wang, Hui;Liu, Xiaoying;...

通讯作者：

Feng, ZM

作者机构：

[He, Yumin; Feng, Zemeng; Wang, Wenjing; Yin, Yulong] Chinese Acad Sci, Hunan Prov Engn Res Ctr Hlth Livestock & Poultry P, Hunan Prov Key Lab Anim Nutr Physiol & Metab Proc, Inst Subtrop Agr,Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Hunan, Peoples R China.

[Liu, Xiaoying; Gui, Qing-wen; Wang, Wenjing] Hunan Agr Univ, Coll Chem & Mat Sci, Changsha 410128, Hunan, Peoples R China.

[He, Yumin] Univ South China, Hengyang Med Sch, Dept Biochem & Mol Biol, Hengyang 421001, Hunan, Peoples R China.

[Deng, Lei] Cent South Univ, Sch Comp Sci & Engn, Changsha 410075, Hunan, Peoples R China.

[Wang, Hui; Xiong, Benhai] Chinese Acad Agr Sci, Inst Anim Sci, State Key Lab Anim Nutr, Beijing 100193, Peoples R China.

通讯机构：

[Feng, ZM ] C

Chinese Acad Sci, Hunan Prov Engn Res Ctr Hlth Livestock & Poultry P, Hunan Prov Key Lab Anim Nutr Physiol & Metab Proc, Inst Subtrop Agr,Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Hunan, Peoples R China.

语种：

英文

关键词：

L-Lys;Polyaniline;Peptide aptamer;Biosensor

期刊：

Measurement

ISSN：

0263-2241

年：

2023

卷：

221

DOI：

10.1016/j.measurement.2023.113468

基金类别：

14th five-year national key research and development plan [2021YFD2000800]; National Key Scientific Instrument and Equipment Development Projects of China [31527803]; Chinese Academy of Science and Technology Service Network Initiative Project [KFJ-STS- QYZD-2021-22-002]; Natural Science Foundation of Hunan Province, China [2021WK4001, 2020JJ40559]; Earmarked Fund for China Agriculture Research System [CARS-35]

机构署名：

本校为其他机构

院系归属：

化学与生物工程学院

摘要：

L-Lysine (L-Lys) quantification in serum using a novel and highly selective amperometric biosensor has been reported. In this study, an efficient, enzyme-free, and simple anti-fouling biosensor was developed based on a self-screened peptide aptamer. A glassy carbon electrode (GCE) was used to construct anti-fouling interfaces by modifying its surface with polyaniline (PANI) polymer. The peptide aptamer, Cys-Pro-Pro-Pro-Pro-Arg-Glu-AsnIle-Gln-Arg-Leu-Thr, was then immobilized onto this electrode via an electroactive cysteine linker and its potential in the determination of L-Lys was examined. U...

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Peptide aptamer-based polyaniline-modified amperometric biosensor for L-lysine detection in real serum samples

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