Changes in Microbial Flora of Fresh-cut Lotus Root during Cold Storage Based on PCR-DGGE Technology
作者：
王发祥;刘永乐;俞健;李向红;王建辉;...
通讯作者：
Liu, Yongle(lyle19@163.com)
作者机构：
[王建辉; 俞健; 刘小芳; 李向红; 刘永乐; 王发祥] College of Chemistry and Biology Engineering, Changsha University of Science and Technology, Hunan Provincial Engineering Research Center for Food Processing of Aquatic Biotic Resources, Changsha, 410114, China
通讯机构：
College of Chemistry and Biology Engineering, Changsha University of Science and Technology, Hunan Provincial Engineering Research Center for Food Processing of Aquatic Biotic Resources, Changsha, China
In order to clear the microbial spoilage mechanism of fresh cut lotus root, changes of its microbial flora during cold storage was investigated using PCR-DGGE technology. The fresh-cut lotus root was refrigerated in 4℃and taken out for amplifying the V3 area of 16s rDNA sequence at every 3 days, then the PCR products were analyzed by DGGE and dominant bands were recovered and sequenced. The results showed that microbial flora of fresh cut lotus root changed obviously during cold storage and 6~9 days was the key point of spoilage. And the main spoilage bacteria of refrigerated lotus root ...
