In this paper, four different methods were used to prepare titania coating for potential biological laser printing technology,, and the substrate surface morphology, roughness, adhesion and biological compatible properties were systematically compared, then the effect of adding surface active agent and light induced hydrophilicity on the surface of the substrate is discussed. The experiment results show that the vacuum evaporation deposition plating method is the best preparation method in in titania substrate of biological laser printing technology. In the biological laser printing protein, t...