The proteins of male - sterile line, its fertile maintainer line and the hybrid F1 were separated by two di- mensional electrophoresis with immobilized gradients as the first dimension and SDS -PAGE as the second, and the high resolution and repeatability 2D maps were obtained. 15 differentially expressed points were identified by MAL- DI-TOF/MS or LC -MS/MS analyses and protein database searching, and then 12points were confirmed. The results showed that some proteins involved in material and energy metabolism, transcription and signal transduction etc. were down - regulated or absent in the ...
