期刊论文

中文

中国粮油学报

1003-0174

2012

27

12

54-61,68

10.3969/j.issn.1003-0174.2012.12.010

湖南省教育厅资助科研项目（11A007）；

本校为第一机构

根据Box-Behnke的中心组合试验设计原理,在单因素试验的基础上,采用四因素三水平的响应面分析法,以出油效率为响应值,考察料液比、酶解pH、酶解时间、酶解温度等因素对超声辅助水酶法提取油茶籽油的影响。结果表明,最佳工艺条件为:酶用量2.62%(蛋白酶∶纤维素酶=4∶1),酶解温度52.1℃,料液比1∶5.58,酶解pH 6.47,超声时间20 min,加热预处理90℃、20 min,酶解时间5 h。在此条件下,出油效率高达83%,蛋白水解率高达90.4%。该工艺制备的油茶籽油无需精炼,各项指标均已符合一级压榨茶油的标准。其中,不饱和脂肪酸质量分数高达87.8%,VE含量为204.5 mg/kg,角鲨烯含量为114.4 mg/kg,在提取过程中完好地保...

The effects of operational variables （ such as water/seeds ratio, hydrolysis pH, hydrolysis time, hydrol- ysis temperature and so on）were studied on the oil extraction yield according to the principle of Box - Behnke. The highest oil recovery of 82.99% was obtained when the seeds were incubated with 1：5.58 of the water/seeds ratio, 2.62% of protease：： cellulose （4：1 ） for 5 h, at 52.1 ℃ and pH 6.47, assisted by hot - pretreatment at 90 ℃for 20 min and the using of uhrasonication for 2 0 rain . Maximum oil yiel...