期刊论文

Gong Fu-Chun

中文

化学学报

0567-7351

2008

66

1

73-78

10.3321/j.issn:0567-7351.2008.01.012

国家自然科学基金（Nos.20775010,50774016）； 湖南省自然科学基金资助项目；

本校为第一且通讯机构

合成了一种新型辣根过氧化物酶（HRP）荧光底物-4-羟基苯乙基吡啶（pHSP），并首次将它运用于酶联荧光免疫传感体系。对pHSP化学性质的研究证实，pHSP在空气中较稳定，对HRP，H_2O_2的荧光响应性能优于传统HRP荧光底物，如对羟苯乙酸、Amplex Red和佳味醇等，在pH5．8的Britton-Robinson缓冲溶液中，pHSP本身只有极弱的荧光，在HRP-IgG催化下可被H2O2氧化成二聚体产物，该二聚体在300nm的激发光下能发射波长为437nm的强荧光，并且反应体系的荧光增加与HRP量在一定浓度范围内成线性相关。根据此原理，建立了兔布氏杆菌抗体的酶联荧光传感分析新方法，运用制备的传感装置测定兔布氏杆菌抗体的线性...

A novel substrate, 4-(p-hydroxystyryl)pyridine (pHSP) was synthesized and firstly used in a horseradish peroxidase (HRP) based fluorometric enzyme-linked immunosensing system. The results of the property investigation of pHSP demonstrate the advantages in stability and reactivity with HRP over conventional substrates such as p-hydroxyphenylacetic acid (p-HPA), Amplex red and chavicol. In a pH 5.8 Britton-Robinson buffer solution, HRP-antibody conjugate (HRP-IgG) could catalyze the oxidation reaction of pHSP by H2O2, and the pHSP was converted to significantly fluorescent dimers. The increase o...