期刊论文

Gong Fuchun

中文

化学学报

0567-7351

2009

67

2

162-166

10.3321/j.issn:0567-7351.2009.02.012

湖南省科技计划项目（No. 2008SK3052）； 湖南省教育厅科研基金（Nos. 08B004, 07A006）资助项目.；

本校为第一且通讯机构

以4-甲氧基-N-(2-N',N'-二甲基氨基乙基-N'-烯丙基)萘二甲酰亚胺氯化铵(DMNAA)为荧光单体,合成了.种pH敏感荧光高分予聚N-异丙基丙烯酰胺-4-甲氧基-N-(2-N-N-二甲基氨基乙基-N'-烯丙基)荼二甲酰亚胺氯化铵-N,N-二甲基氨内基甲基内烯酰胺[P(NIP-DMAPM-DMNAA)].采用共聚法将同本血吸虫抗原(SjAg)固定在P(NIP-DMAPM-DMNAA)上,制备P(NIP-DMAPM-DMNAA)-SjAg连接物,与日本血吸虫抗体(待测,SjAb)发生免疫反应后,调节 pH 值,使荧光高分子相变分离高分子一免疫组分连接物,最后,利用蛋白 A对抗体的亲和性捕获P(NIP-DMAPM-DMNAA)-SjAg-SjAb,通过测定高分子自身的荧光信号米定量SjAb.该新型高分子具有良好的...

A phase-separation immunoassay method for Schistosomia japonicum antibody (SjAb) was developed using a pH-sensitive fluorescent polymer prepared by polymerization of N-isopropylacrylamide (NIP), N-(3-dimethylaminopropyl)methacrylamide (DMAPM) and 4-methoxy-N-(2-N', N'-dimethylaminoethyl- N'-allyl)naphthalimide chloride quaternary ammonium salt (DMNAA) as a carrier and indicator. The polymer P(NIP-DMAPM-DMNAA) possesses relatively strong fluorescence and can be precipitated out of water above a critical pH 7.2 and re-dissolved below pH 7.2. The characteristic of this "smart" polymer made it pos...