The Gastrodia tuber and its active component, gastrodin, have many pharmacological effects. In this study, optimized high performance liquid chromatography (HPLC) parameters were employed to determine the chemical fingerprints and gastrodin content of nine Gastrodia tuber populations. Based on the degree of similarity of the chemical fingerprints, the nine Gastrodia tuber populations were grouped into one of the three different classes. Class I Gastrodia tubers had the highest content of gastrodin and were thus, regarded as possessing the highe...