A Target-Lighted dsDNA-Indicator for High-Performance Monitoring of Mercury Pollution and Its Antagonists Screening
作者：
Qing, Zhihe* ;Zhu, Lixuan;Li, Xiaoxuan;Yang, Sheng;Zoup, Zhen;...
期刊：
Environmental Science & Technology ,2017年51(20):11884-11890 ISSN：0013-936X
通讯作者：
Qing, Zhihe;Yang, Ronghua
作者机构：
[Li, Xiaoxuan; Yang, Sheng; Guo, Jingru; Yang, Ronghua; Zoup, Zhen; Qing, Zhihe; Zhu, Lixuan; Cao, Zhong] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Hunan Prov Engn Res Ctr Food Proc Aquat Biot Reso, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Changsha 410114, Hunan, Peoples R China.;[Yang, Sheng; Yang, Ronghua; Zoup, Zhen; Qing, Zhihe] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Coll Chem & Chem Engn, Mol Sci & Biomed Lab, Changsha 410082, Hunan, Peoples R China.
通讯机构：
[Qing, ZH; Yang, RH] C;[Qing, ZH; Yang, RH] H;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Hunan Prov Engn Res Ctr Food Proc Aquat Biot Reso, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Changsha 410114, Hunan, Peoples R China.;Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Coll Chem & Chem Engn, Mol Sci & Biomed Lab, Changsha 410082, Hunan, Peoples R China.
摘要：
As well-known, the excessive discharge of heavy-metal mercury not only destroys the ecological environment, bust also leads to severe damage of human health after ingestion via drinking and bioaccumulation of food chains, and mercury ion (Hg2+) is designated as one of most prevalent toxic metal ions in drinking water. Thus, the high-performance monitoring of mercury pollution is necessary. Functional nucleic acids have been widely used as recognition probes in biochemical sensing. In this work, a carbazole derivative, ethyl-4-[3,6-bis(1-methyl-4-vinylpyridium iodine)-9H-carbazol -9-yl)] butanoate (EBCB), has been synthesized and found as a target-lighted DNA fluorescent indicator. As a proof-of-concept, Hg2+ detection was carried out based on EBCB and Hg2+-mediated conformation transformation of a designed DNA probe. By comparison with conventional nucleic acid indicators, EBCB held excellent advantages, such as minimal background interference and maximal sensitivity. Outstanding detection capabilities were displayed, especially including simple operation (add-and-read manner), ultrarapidity (30 s), and low detection limit (0.82 nM). Furthermore, based on these advantages, the potential for high-performance screening of mercury antagonists was also demonstrated by the fluorescence change of EBCB. Therefore, we believe that this work is meaningful in pollution monitoring, environment restoration and emergency treatment, and may pave a way to apply EBCB as an ideal signal transducer for development of high-performance sensing strategies. © 2017 American Chemical Society.
语种：
英文
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Direct Detection of Nucleic Acid with Minimizing Background and Improving Sensitivity Based on a Conformation-Discriminating Indicator
作者：
Zhu, Lixuan;Qing, Zhihe* ;Hou, Lina;Yang, Sheng;Zou, Zhen;...
期刊：
ACS SENSORS ,2017年2(8):1198-1204 ISSN：2379-3694
通讯作者：
Qing, Zhihe;Yang, Ronghua
作者机构：
[Yang, Sheng; Hou, Lina; Zou, Zhen; Yang, Ronghua; Zhu, Lixuan; Qing, Zhihe; Cao, Zhong] Changsha Univ Sci & Technol, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Hunan Prov Engn Res Ctr Food Proc Aquat Biot Reso, Sch Chem & Biol Engn, Changsha 410114, Hunan, Peoples R China.;[Yang, Ronghua; Qing, Zhihe] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Mol Sci & Biomed Lab, Changsha 410082, Hunan, Peoples R China.
通讯机构：
[Qing, ZH; Yang, RH] C;[Qing, ZH; Yang, RH] H;Changsha Univ Sci & Technol, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Hunan Prov Engn Res Ctr Food Proc Aquat Biot Reso, Sch Chem & Biol Engn, Changsha 410114, Hunan, Peoples R China.;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Mol Sci & Biomed Lab, Changsha 410082, Hunan, Peoples R China.
关键词：
nucleic acid;sensitive;direct detection;fluorescent;indicator
摘要：
As is well-known, the nucleic acid indicator-based strategy is one of the major approaches to monitor the nucleic acid hybridization-mediated recognition events in biochemical analysis, displaying obvious advantages including simplicity, low cost, convenience, and generality. However, conventional indicators either hold strong self-fluorescence or can be lighted by both ssDNA and dsDNA, lacking absolute selectivity for a certain conformation, always with high background interference and low sensitivity in sensing; and additional processing (e.g., nanomaterial-mediated background suppression, and enzyme-catalyzed signal amplification) is generally required to improve the detection performance. In this work, a carbazole derivative, EBCB, has been synthesized and screened as a dsDNA-specific fluorescent indicator. Compared with conventional indicators under the same conditions, EBCB displayed a much higher selective coefficient for dsDNA, with little self-fluorescence and negligible effect from ssDNA. Based on its superior capability in DNA conformation-discrimination, high sensitivity with minimizing background interference was demonstrated for direct detection of nucleic acid, and monitoring nucleic acid-based circuitry with good reversibity, resulting in low detection limit and high capability for discriminating base-mismatching. Thus, we expect that this highly specific DNA conformation-discriminating indicator will hold good potential for application in biochemical sensing and molecular logic switching. © 2017 American Chemical Society.
语种：
英文
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Noninvasive and Highly Selective Monitoring of Intracellular Glucose via a Two-Step Recognition-Based Nanokit
作者：
Tang, Jianru;Ma, Dandan;Pecic, Stevan;Huang, Caixia;Zheng, Jing* ;...
期刊：
Analytical Chemistry ,2017年89(16):8319-8327 ISSN：0003-2700
通讯作者：
Zheng, Jing;Yang, Ronghua
作者机构：
[Huang, Caixia; Zheng, Jing; Yang, Ronghua; Zheng, J; Tang, Jianru; Ma, Dandan; Li, Jishan] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Yang, Ronghua] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410082, Hunan, Peoples R China.;[Pecic, Stevan] Columbia Univ, Univ Med Ctr, New York, NY 10032 USA.
通讯机构：
[Zheng, J; Yang, RH] H;[Yang, Ronghua] C;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410082, Hunan, Peoples R China.
摘要：
Accurate determination of intracellular glucose is very important for exploring its chemical and biological functions in metabolism events of living cells. In this paper, we developed a new noninvasive and highly selective nanokit for intracellular glucose monitoring via two-step recognition. The liposome-based nanokit coencapsulated the aptamer-functionalized gold nanoparticles (AuNPs) and the Shinkai's receptor together. When the proposed nanokit was transfected into living cells, the Shinkai's receptor could recognize glucose first and then changed its conformation to endow aptamers with binding and sensing properties which were not readily accessible otherwise. Then, the binary complexes formed by the intracellular glucose and the Shinkai's receptor can in situ displace the complementary oligonucleotide of the aptamer on the surface of AuNPs. The fluorophore-labeled aptamer was away from the AuNPs, and the fluorescent state switched from "off" to "on". Through the secondary identification of aptamer, the selectivity of the Shinkai's receptor could be greatly improved while the intracellular glucose level was assessed by fluorescence signal recovery of aptamer. In the follow-up application, the approach exhibits excellent selectivity and is noninvasive for intracellular glucose monitoring under normoxia and hypoxia. To the best of our knowledge, this is the first time that the advantages of organic receptors and nucleic acids have been combined and highly selective monitoring of intracellular glucose has been realized via two-step recognition. We expect it to open up new possibilities to integrate devices for diagnosis of various metabolic diseases and insulin delivery. © 2017 American Chemical Society.
语种：
英文
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A TP-FRET-based two-photon fluorescent probe for ratiometric visualization of endogenous sulfur dioxide derivatives in mitochondria of living cells and tissues
作者：
Yang, Xiaoguang;Zhou, Yibo;Zhang, Xiufang;Yang, Sheng* ;Chen, Yun;...
期刊：
Chemical Communications ,2016年52(67):10289-10292 ISSN：1359-7345
通讯作者：
Yang, Sheng;Yang, Ronghua
作者机构：
[Yang, Sheng; Li, Xiaoxuan; Yang, Ronghua; Yang, Xiaoguang; Guo, Jingru; Qing, Zhihe] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410114, Hunan, Peoples R China.;[Zhang, Xiufang; Yang, Ronghua; Zhou, Yibo; Chen, Yun] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Coll Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China.
通讯机构：
[Yang, S; Yang, RH] C;[Yang, Ronghua] H;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410114, Hunan, Peoples R China.;Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Coll Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China.
摘要：
A ratiometric two-photon fluorescent probe for SO2 derivatives was first proposed based on acedan-merocyanine dyads via a TP-FRET strategy. It was successfully applied to visualization of the fluctuations of enzymatically generated SO2 derivatives in the mitochondria of HepG2 cells and rat liver tissues using two-photon fluorescence microscopy imaging.
语种：
英文
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A Reversible Nanolamp for Instantaneous Monitoring of Cyanide Based on an Elsner-Like Reaction
作者：
Qing, Zhihe;Hou, Lina;Yang, Le;Zhu, Lixuan;Yang, Sheng;...
期刊：
Analytical Chemistry ,2016年88(19):9759-9765 ISSN：0003-2700
通讯作者：
Yang, Ronghua
作者机构：
[Yang, Sheng; Hou, Lina; Yang, Ronghua; Qing, Zhihe; Zhu, Lixuan] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410114, Hunan, Peoples R China.;[Yang, Le; Yang, Ronghua; Zheng, Jing; Qing, Zhihe] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Coll Chem & Chem Engn, Mol Sci & Biomed Lab, Changsha 410082, Hunan, Peoples R China.
通讯机构：
[Yang, Ronghua] C;[Yang, Ronghua] H;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410114, Hunan, Peoples R China.;Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Coll Chem & Chem Engn, Mol Sci & Biomed Lab, Changsha 410082, Hunan, Peoples R China.
摘要：
It is well-known that cyanide ion (CN-) is a hypertoxic anion, which can cause adverse effects in both the environment and living beings; thus, it is highly desirable to develop strategies for detecting CN-, especially in water and food. However, due to the short half-life of free cyanide, long analysis time and/or interference from other competitive ions are general challenges for accurate monitoring of CN-. In this work, through the investigation on the sequence-dependent optical interaction of DNA-CuNPs with the fluorophore (e.g., EBMVC-B), we found, for the first time, that DNA-CuNPs were an ideal alternative as fluorescence quencher in constructing a sensor which could be illuminated by CN- based on an Elsner-like reaction and that the signal switching was dependent on poly(AT/TA) dsDNA sequence. By virtue of CuNPs' small size and its high chemical reactivity with cyanide, the lighting of fluorescence was ultrarapid and similar to the hairtrigger "turn-on" of a lamp, which is significant for accurately monitoring a target of short half-life (e.g., cyanide). Attributed to the unique Elsner-like reaction between CN- and the Cu atoms, high selectivity was achieved for CN- monitoring by the nanolamp, with practical applications in real water and food samples. In addition, because of the highly efficient in situ formation of DNA-CuNPs and the approximative stoichiometry between CN- and Cu2+ in the fluorescence switching, the nanolamp could be reversibly turned on and off through the alternate regulation of CN- and Cu2+, displaying potential for developing reusable nanosensors and constructing optical molecular logic circuits. © 2016 American Chemical Society.
语种：
英文
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SERS monitoring the dynamics of local pH in lysosome of living cells during photothermal therapy
作者：
Luo, Rongxing;Li, Yinhui;Zhou, Qifeng;Zheng, Jing* ;Ma, Dandan;...
期刊：
ANALYST ,2016年141(11):3224-3227 ISSN：0003-2654
通讯作者：
Zheng, Jing;Yang, Ronghua
作者机构：
[Luo, Rongxing; Tang, Pinting; Zheng, Jing; Yang, Ronghua; Zheng, J; Li, Yinhui; Ma, Dandan; Zhou, Qifeng] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Yang, Sheng; Yang, Ronghua; Qing, Zhihe] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Zheng, J; Yang, RH] H;[Yang, Ronghua] C;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.
摘要：
In this communication, we propose a gold nanorod-based SERS nanotracker to monitor the local pH change during photothermal therapy (PTT). The dynamic SERS analysis indicated that the local pH of the lysosome in living cells increased during the PTT which would be helpful to understand the cellular metabolic processes and further facilitate the photosensitizer screening and optimization.
语种：
英文
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Target-Activated Modulation of Dual-Color and Two-Photon Fluorescence of Graphene Quantum Dots for in Vivo Imaging of Hydrogen Peroxide
作者：
Zhao, Wenjie;Li, Yinhui;Yang, Sheng* ;Chen, Yun;Zheng, Jing;...
期刊：
Analytical Chemistry ,2016年88(9):4833-4840 ISSN：0003-2700
通讯作者：
Yang, Ronghua;Yang, Sheng
作者机构：
[Liu, Changhui; Zhao, Wenjie; Zheng, Jing; Yang, Ronghua; Chen, Yun; Li, Yinhui; Li, Jishan] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Liu, Changhui; Zhao, Wenjie; Zheng, Jing; Yang, Ronghua; Chen, Yun; Li, Yinhui; Li, Jishan] Hunan Univ, Collaborat Innovat Ctr Chem & Mol Med, Changsha 410082, Hunan, Peoples R China.;[Yang, Sheng; Yang, Ronghua; Qing, Zhihe] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Yang, Ronghua] H;[Yang, S; Yang, RH] C;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;Hunan Univ, Collaborat Innovat Ctr Chem & Mol Med, Changsha 410082, Hunan, Peoples R China.;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.
摘要：
The development of nanoprobes suitable for two-photon microscopy techniques is highly desirable for mapping biological species in living systems. However, at the current stage, the nanoprobes are restricted to single-color fluorescence changes, making it unsuitable for quantitative detection. To circumvent this problem, we report here a rational design of a dual-emission and two-photon (TP) graphene quantum dot (GQD420) probe for imaging of hydrogen peroxide (H2O2). For specific recognition of H2O2 and lighting the fluorescence of TPGQD420, a boronate ester-functionalized merocyanine (BMC) fluorophore was used as both target-activated trigger and the dual-emission fluorescence modulator. Upon two-photon excitation at 740 nm, TPGQD420-BMC displays a green-to-blue resolved emission band in response to H2O2 with an emission shift of 110 nm, and the H2O2 can be determined from 0.2 to 40 μM with a detection limit of 0.05 μM. Moreover, the fluorescence response of the TPGQD420-BMC toward H2O2 is rapid and extremely specific. The feasibility of the proposed method is demonstrated by two-photon ratiometrically mapping the production of endogenous H2O2 in living cells as well as in deep tissues of murine mode at 0-600 μm. To the best of our knowledge, this is the first paradigm to rationally design a dual-emission and two-photon nanoprobe via fluorescence modulation of GQDs with switchable molecules, which will extend new possibility to design powerful molecular tools for in vivo bioimaging applications. © 2016 American Chemical Society.
语种：
英文
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Fabrication of an electrochemical sensor based on spiropyran for sensitive and selective detection of fluoride ion
作者：
Tao, Jia;Zhao, Peng;Li, Yinhui* ;Zhao, Wenjie;Xiao, Yue;...
期刊：
Analytica Chimica Acta ,2016年918:97-102 ISSN：0003-2670
通讯作者：
Yang, Ronghua;Li, Yinhui
作者机构：
[Yang, Ronghua; Tao, Jia] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.;[Xiao, Yue; Zhao, Wenjie; Yang, Ronghua; Tao, Jia; Li, YH; Li, Yinhui; Zhao, Peng] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Tao, Jia] S China Univ Technol, Coll Chem Sci & Engn, Guangzhou 510641, Guangdong, Peoples R China.
通讯机构：
[Yang, Ronghua] C;[Li, YH; Yang, RH] H;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.
关键词：
Electrochemical sensors;Fluoride ion;SWCNTs;Spiropyran
摘要：
In the past decades, numerous electrochemical sensors based on exogenous electroactive substance have been reported. Due to non-specific interaction between the redox mediator and the target, the instability caused by false signal may not be avoided. To address this issue, in this paper, a new electrochemical sensor based on spiropyran skeleton, namely SPOSi, was designed for specific electrochemical response to fluoride ions (F-). The breakage of Si-O induced by F- based on the specific nucleophilic substitution reaction between F- and silica would directly produce a hydroquinone structure for electrochemical signal generation. To improve the sensitivity, SPOSi probe was assembled on the single-walled carbon nanotubes (SWCNTs) modified glassy carbon electrode (GCE) through the π-π conjugating interaction. This electrode was successfully applied to monitor F- with a detection limit of 8.3 × 10-8 M. Compared with the conventional F- ion selected electrode (ISE) which utilized noncovalent interaction, this method displays higher stability and a comparable sensitivity in the urine samples. © 2016 Elsevier B.V.
语种：
英文
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MWCNTs-rGO/PDDA-AuNPs nanocomposite modified electrode for sensitive detection of ractopamine
作者：
Xun Yan;Cao Zhong* ;Song Tianming;Lu Chaozhi;Liu Feng;...
期刊：
高等学校化学学报 ,2016年37(5):835-843 ISSN：0251-0790
通讯作者：
Cao Zhong
作者机构：
[Yang Ronghua; Liu Feng; He Jinglin; Cao Zhong; Song Tianming; Xun Yan; Lu Chaozhi] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Collaborat Innovat Ctr Micro Nano Biosensing & Fo, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Changsha 410114, Hunan, Peoples R China.
通讯机构：
[Cao Zhong] C;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Collaborat Innovat Ctr Micro Nano Biosensing & Fo, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Changsha 410114, Hunan, Peoples R China.
关键词：
莱克多巴胺;还原型氧化石墨烯;聚二烯丙基二甲基氯化铵;MWCNTs-rGO/PDDA-AuNPs复合物;食品安全检测
摘要：
采用自组装方法,将聚二烯丙基二甲基氯化铵(PDDA)功能化的金纳米颗粒(AuNPs)负载于多壁碳纳米管(MWCNTs)-还原型氧化石墨烯(rGO)夹层,再涂覆于玻碳电极(GCE)上,制备了纳米复合膜修饰电极MWCNTs-rGO/PDDA-AuNPs /GCE. 采用透射电子显微镜(TEM)和紫外-可见光谱(UV-Vis)对修饰膜的形貌及结构进行表征. 探讨了其对莱克多巴胺(Rac)的循环伏安行为,结果表明MWCNTs-rGO/PDDA-AuNPs纳米复合物对Rac表现出显著的电催化氧化特性. 采用差分脉冲伏安法测得该复合膜修饰电极对Rac检测的线性范围为0.036 ~ 4.5 μmol /L,检出限为6.35 nmol /L(S /N≥3),且显示出良好的抗干扰能力、稳定性及重现性. 采用该方法检测猪血清及猪尿样中的Rac,回收率达95.4%~ 105.9%,表明该复合膜修饰电极对实际样品中Rac的检测具有潜在应用价值.
语种：
中文
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双控激活式亚细胞区域定位荧光探针设计
作者：
杨荣华;杨盛;李银辉;赵一蓉;伍伟
作者机构：
[杨荣华; 杨盛; 李银辉; 赵一蓉; 伍伟] 长沙理工大学化学与生物工程学院;[杨荣华; 杨盛; 李银辉; 赵一蓉; 伍伟] 湖南大学化学化工学院
会议名称：
中国化学会第30届学术年会
会议时间：
2016-01-01
会议地点：
中国辽宁大连
会议论文集名称：
中国化学会第30届学术年会摘要集-第四分会:生物分析和生物传感
关键词：
亚细胞区域定位;双控激活式;荧光探针;生命活性物质
摘要：
准确高效地获取亚细胞层面上的化学生物信息,对于理解生命活性物质在亚细胞结构中的分子作用机制,探究生命活性物质与细胞器两者之间的相互影响关系具有重要的意义[1]。发展能够精准定位于亚细胞区域的荧光探针成为近年来的研究热点[2]。亚细胞区域定位荧光探针的常规设计策略是在探针分子上修饰导向基团,将其靶向到特定细胞器[3]。然而导向基团的靶向能力并非尽善尽美,而分析对象又非特异性分布在细
语种：
中文
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Visual Biopsy by Hydrogen Peroxide-Induced Signal Amplification
作者：
Zhao, Wenjie;Yang, Sheng;Yang, Jinfeng;Li, Jishan;Zheng, Jing;...
期刊：
Analytical Chemistry ,2016年88(21):10728-10735 ISSN：0003-2700
通讯作者：
Yang, Ronghua
作者机构：
[Zhao, Wenjie; Zheng, Jing; Yang, Ronghua; Li, Jishan] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Zhao, Wenjie; Zheng, Jing; Yang, Ronghua; Li, Jishan] Hunan Univ, Collaborat Innovat Ctr Chem & Mol Med, Changsha 410082, Hunan, Peoples R China.;[Yang, Sheng; Yang, Ronghua; Qing, Zhihe] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410114, Hunan, Peoples R China.;[Yang, Jinfeng] Cent S Univ, Xiangya Sch Med, Affiliated Canc Hosp, Changsha 410011, Hunan, Peoples R China.
通讯机构：
[Yang, Ronghua] H;[Yang, Ronghua] C;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;Hunan Univ, Collaborat Innovat Ctr Chem & Mol Med, Changsha 410082, Hunan, Peoples R China.;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410114, Hunan, Peoples R China.
摘要：
Visual biopsy has attracted special interest by surgeons due to its simplicity and practicality; however, the limited sensitivity of the technology makes it difficult to achieve an early diagnosis. To circumvent this problem, herein, we report a visual signal amplification strategy for establishing a marker-recognizable biopsy that enables early cancer diagnosis. In our proposed approach, hydrogen peroxide (H2O2) was selected as a potential underlying marker for its compact relationship in cancer progression. For selective recognition of H2O2 in the process of visual biopsy, a benzylbenzeneboronic acid pinacol ester-decorated copolymer, namely, PMPC-Bpe, was synthesized, affording the final formation of the H2O2-responsive micelles in which amylose was trapped. The presence of H2O2 activates the boronate ester recognition site and induces it releasing abundant indicator amylose, leading to signal amplification. The indicator came across the solution of KI/I2 added to the sample, and the formative amylose-KI/I2 complex has a distinct blue color at 574 nm for visual amplification detection. The feasibility of the proposed method is demonstrated by visualizing the H2O2 content of cancer at different stages and three kinds of actual cancerous samples. As far as we know, this is the first paradigm to rationally design a signaling amplification-based molecular recognizable biopsy for visual and sensitive disease identification, which will extend new possibilities for marker-recognition and signal amplification-based biopsy in disease progressing. © 2016 American Chemical Society.
语种：
英文
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Detection of Circulating Tumor DNA in Human Blood via DNA-Mediated Surface-Enhanced Raman Spectroscopy of Single-Walled Carbon Nanotubes
作者：
Zhou, Qifeng;Zheng, Jing* ;Qing, Zhihe;Zheng, Mengjie;Yang, Jinfeng;...
期刊：
Analytical Chemistry ,2016年88(9):4759-4765 ISSN：0003-2700
通讯作者：
Zheng, Jing;Yang, Ronghua
作者机构：
[Zheng, Jing; Zheng, Mengjie; Yang, Jinfeng; Ying, Le; Zhou, Qifeng] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Yang, Sheng; Yang, Ronghua; Qing, Zhihe] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Zheng, Jing] H;[Yang, Ronghua] C;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.
摘要：
The levels of circulating tumor DNA (ctDNA) in the peripheral blood have been associated with tumor burden and malignant progression. However, ultrasensitive detection of ctDNA in blood remains to be explored. Herein, we have developed a new approach, employing DNA-mediated surface-enhanced Raman scattering (SERS) of single-walled carbon nanotubes (SWNTs), that allows ultrasensitive detection of a broad range of ctDNAs in human blood. Combined with the efficient ctDNA recognition capacity of our designed triple-helix molecular switch and RNase HII enzyme-assisted amplification, the T-rich DNA-mediated SERS enhancement of SWNTs could read out a content of KRAS G12DM as low as 0.3 fM, with a detection of 5.0 μL of sample volume, which has potential for point-of-care testing in clinical analysis. © 2016 American Chemical Society.
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英文
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Engineering a nanolab for the determination of lysosomal nitric oxide by the rational design of a pH-activatable fluorescent probe
作者：
Li, Yinhui* ;Wu, Wei;Yang, Jinfeng;Yuan, Lin;Liu, Changhui;...
期刊：
CHEMICAL SCIENCE ,2016年7(3):1920-1925 ISSN：2041-6520
通讯作者：
Li, Yinhui;Yang, Ronghua
作者机构：
[Liu, Changhui; Yuan, Lin; Zheng, Jing; Yang, Ronghua; Wu, Wei; Li, YH; Li, Yinhui] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Yang, Ronghua] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.;[Yang, Jinfeng] Cent S Univ, Xiangya Sch Med, Tumor Hosp, Changsha 410013, Hunan, Peoples R China.
通讯机构：
[Li, YH; Yang, RH] H;[Yang, Ronghua] C;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.
摘要：
Nitric oxide (NO) is often involved in many different physiological processes including the regulation of lysosomal functions. However, it remains a great challenge to explore the variations of NO levels in lysosomes, limiting the understanding behind its biological functions in cellular signaling pathways and various diseases. Herein, a pH-activatable fluorescent probe, Rhod-H-NO, was designed and synthesized for the determination of lysosomal NO, in which the activation response model is beneficial towards getting accurate biological information. To ensure that Rhod-H-NO can accumulate effectively and exist stably in lysosomes without interference and degradation from other active species, Rhod-H-NO was engineered into the nanopores of mesoporous silica nanoparticles (MSNs) with β-cyclodextrin (β-CD) as the gatekeeper to obtain a nanolab. The nanolab was successfully applied to detect lysosomal NO in living cells and in vivo with high time and spatial resolution. This nanolab could serve as an excellent molecular tool to exploit and elucidate the function of NO at sub-cellular levels. © The Royal Society of Chemistry 2016.
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英文
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SERS assay of telomerase activity at single-cell level and colon cancer tissues via quadratic signal amplification
作者：
Shi, Muling;Zheng, Jing* ;Liu, Changhui;Tan, Guixiang;Qing, Zhihe;...
期刊：
Biosensors and Bioelectronics ,2016年77:673-680 ISSN：0956-5663
通讯作者：
Zheng, Jing
作者机构：
[Liu, Changhui; Yang, Sheng; Tan, Yongjun; Tan, Guixiang; Shi, Muling; Zheng, Jing; Yang, Ronghua; Qing, Zhihe] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Coll Biol, Changsha 410082, Hunan, Peoples R China.;[Yang, Ronghua] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.;[Yang, Jinfeng] Cent South Univ, Xiangya Sch Med, Affiliated Canc Hosp, Dept Anesthesiol, Changsha 410013, Hunan, Peoples R China.;[Zheng, Jing] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.
通讯机构：
[Zheng, Jing] H;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.
关键词：
Cancer tissue;Quadratic amplification;Silver nanoparticle (AgNP);Surface-enhance Raman Spectroscopy;Telomerase activity
摘要：
As an important biomarker and therapeutic target, telomerase has attracted extensive attention concerning its detection and monitoring. Recently, enzyme-assisted amplification approaches have provided useful platforms for the telomerase activity detection, however, further improvement in sensitivity is still hindered by the single-step signal amplification. Herein, we develop a quadratic signal amplification strategy for ultrasensitive surface-enhanced Raman scattering (SERS) detection of telomerase activity. The central idea of our design is using telomerase-induced silver nanoparticles (AgNPs) assembly and silver ions (Ag+)-mediated cascade amplification. In our approach, each telomerase-aided DNA sequence extension could trigger the formation of a long double-stranded DNA (dsDNA), making numerous AgNPs assembling along with this long strand through specific Ag-S bond, to form a primary amplification element. For secondary amplification, each conjugated AgNP was dissolved into Ag+, which can effectively induce the 4-aminobenzenethiol (4-ABT) modified gold nanoparticles (AuNPs at 4-ABT) to undergo aggregation to form numerous "hot-spots". Through quadratic amplifications, a limit of detection down to single HeLa cell was achieved. More importantly, this method demonstrated good performance when applied to tissues from colon cancer patients, which exhibits great potential in the practical application of telomerase-based cancer diagnosis in early stages. To demonstrate the potential in screening the telomerase inhibitors and telomerase-targeted drugs, the proposed design is successfully employed to measure the inhibition of telomerase activity by 3'-azido-3'-deoxythymidine. © 2015 Elsevier B.V.
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英文
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SERS Amplification Assays of Nucleic Acids and Proteins with Functional nanomaterials
作者：
Ronghua Yang;Jing Zheng;Muling Shi;Qifeng Zhou
作者机构：
[Ronghua Yang; Jing Zheng; Muling Shi; Qifeng Zhou] School of Chemistry and Biological Engineering, Changsha University of Science and Technology;[Ronghua Yang; Jing Zheng; Muling Shi; Qifeng Zhou] College of Chemistry and Chemical Engineering, Hunan University
会议名称：
中国化学会第30届学术年会
会议时间：
2016-01-01
会议地点：
中国辽宁大连
会议论文集名称：
中国化学会第30届学术年会摘要集-第三分会:纳米传感新原理新方法
关键词：
Surface-enhanced Raman scattering;Functional nanomaterials;Biosensing
摘要：
Surface-enhanced Raman scattering（SERS） has become one of the most valuable tools in chemistry, biology, and materials science.1 In particular, by integrating the extremely strong Raman enhancement of metallic substrates and the highly specific recognition ability of biomolecules, SERS has been used to create many novel bioanalytical tools, such as DNA sequence detection, protein assay, and bacterial or pathogen identification.2 In the recent years, we aim to develop SERS amplification assays of nucleic acids and proteins with functional nanomaterials, which include:1) Combing with DNA nanomachine-functionalized silver nanoparticles to construct reversible and regenerable SERS-active substrate, and thus attain ultrasensitive biomolecules sensing.3-5 2) Combing with nanomaterials, such as single-walled carbon nanotube, to achieve micro RNA and ATP detection.6-7 3) Combing with ions-mediated cascade amplification, thus realize single nucleotide polymorphism and telomerase detection.8 These new SERS amplification assays might open up new opportunities in the application of functional nucleic acid in biosensing.
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英文
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In Vivo Lighted Fluorescence via Fenton Reaction: Approach for Imaging of Hydrogen Peroxide in Living Systems
作者：
Liu, Changhui;Chen, Weiju;Qing, Zhihe* ;Zheng, Jing;Xiao, Yue;...
期刊：
Analytical Chemistry ,2016年88(7):3998-4003 ISSN：0003-2700
通讯作者：
Qing, Zhihe;Yang, Ronghua
作者机构：
[Liu, Changhui; Yang, Sheng; Yang, Ronghua; Qing, Zhihe] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.;[Liu, Changhui] Hunan City Univ, Dept Chem & Environm Engn, Yiyang 413000, Peoples R China.;[Liu, Changhui; Wang, Lili; Xiao, Yue; Yang, Ronghua; Zheng, Jing; Li, Yinhui; Chen, Weiju] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.
通讯机构：
[Qing, ZH; Yang, RH] C;[Yang, Ronghua] H;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.
摘要：
By virtue of its high sensitivity and rapidity, Fenton reaction has been demonstrated as a powerful tool for in vitro biochemical analysis; however, in vivo applications of Fenton reaction still remain to be exploited. Herein, we report, for the first time, the design, formation and testing of Fenton reaction for in vivo fluorescence imaging of hydrogen peroxide (H2O2). To realize in vivo fluorescence imaging of H2O2 via Fenton reaction, a functional nanosphere, Fc@MSN-FDNA/PTAD, is fabricated from mesoporous silica nanoparticle (MSN), a Fenton reagent of ferrocene (Fc), ROX-labeled DNA (FDNA), and a cationic perylene derivative (PTAD). The ferrocene molecules are locked in the pore entrances of MSN, and exterior of MSN is covalently immobilized with FDNA. As a key part, PTAD acts as not only the gatekeeper of MSN but also the efficient quencher of ROX. H2O2 can permeate into the nanosphere and react with ferrocene to product hydroxyl radical (·OH) via Fenton reaction, which cleaves FDNA to detach ROX from PTAD, thus in turn, lights the ROX fluorescence. Under physiological condition, H2O2 can be determined from 5.0 nM to 1.0 μM with a detection limit of 2.4 nM. Because of the rapid kinetics of Fenton reaction and high specificity for H2O2, the proposed method meets the requirement for real applications. The feasibility of Fc@MSN-FDNA/PTAD for in vivo applications is demonstrated for fluorescence imaging of exogenous and endogenous H2O2 in cells and mice. We expect that this work will not only contribute to the H2O2-releated studies but also open up a new way to exploit in vivo Fenton reaction for biochemical research. (Chemical Equation Presented). © 2016 American Chemical Society.
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英文
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Quantitative monitoring of hypoxia-induced intracellular acidification in lung tumor cells and tissues using activatable surface-enhanced raman scattering nanoprobes
作者：
Ma, Dandan;Zheng, Jing* ;Tang, Pinting;Xu, Weijian;Qing, Zhihe;...
期刊：
Analytical Chemistry ,2016年88(23):11852-11859 ISSN：0003-2700
通讯作者：
Zheng, Jing;Yang, Ronghua
作者机构：
[Xu, Weijian; Tang, Pinting; Zheng, Jing; Yang, Ronghua; Zheng, J; Ma, Dandan; Li, Jishan] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Yang, Sheng; Yang, Ronghua; Qing, Zhihe] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Zheng, J; Yang, RH] H;[Yang, Ronghua] C;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.
摘要：
Hypoxia is considered to contribute to pathophysiology in various cells and tissues, and a clear understanding about the relationship between hypoxia and intracellular acidification will help to elucidate the complex mechanism of glycolysis under hypoxia. However, current studies are mainly focused on overexpression of intracellular reductases accelerated by hypoxia, and the investigations focusing on the relationship between hypoxic degree and intracellular acidification remain to be explored. For this vacuity, we report herein a new activatable nanoprobe for sensing pH change under different degrees of hypoxia by surface-enhanced Raman spectroscopy (SERS). The monitoring was based on the SERS spectra changes of 4- nitrothiophenol (4-NTP)-functionalized gold nanorods (AuNR@4- NTP) resulting from the nitroreductase (NTR)-triggered reduction under hypoxic conditions while the as-generated 4-aminothiophenol (4-ATP) is a pH-sensitive molecule. This unique property can ensure the SERS monitoring of intracellular acidification in living cells and tissues under hypoxic conditions. Dynamic pH analysis indicated that the pH decreased from 7.1 to 6.5 as a function of different degrees of hypoxia (from 15 to 1%) due to excessive glycolytic activity triggered by hypoxia. Given the known advantages of SERS sensing, these findings hold promise in studies of pathophysiological pathways involving hypoxia. © 2016 American Chemical Society.
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英文
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Direct Fluorescent Detection of Blood Potassium by Ion-Selective Formation of Intermolecular G-Quadruplex and Ligand Binding
作者：
Yang, Le;Qing, Zhihe* ;Liu, Changhui;Tang, Qiao;Li, Jishan;...
期刊：
Analytical Chemistry ,2016年88(18):9285-9292 ISSN：0003-2700
通讯作者：
Yang, Ronghua;Qing, Zhihe
作者机构：
[Liu, Changhui; Yang, Le; Zheng, Jing; Yang, Ronghua; Tang, Qiao; Li, Jishan; Tan, Weihong] Hunan Univ, Coll Chem & Chem Engn, Mol Sci & Biomed Lab, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Yang, Sheng; Yang, Ronghua; Qing, Zhihe] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Yang, Ronghua] H;[Qing, ZH; Yang, RH] C;Hunan Univ, Coll Chem & Chem Engn, Mol Sci & Biomed Lab, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.
摘要：
G-quadruplex analogues have been widely used as molecular tools for detection of potassium ion (K+). However, interference from a higher concentration of sodium ion (Na+), enzymatic degradation of the oligonucleotide, and background absorption and fluorescence of blood samples have all limited the use of G-quadruplex for direct detection of K+ in blood samples. Here, we reported, for the first time, an intermolecular G-quadruplex-based assay capable of direct fluorescent detection of blood K+. Increased stringency of intermolecular G-quadruplex formation based on our screened G-rich oligonucleotide (5′-TGAGGGA GGGG-3′) provided the necessary selectivity for K+ against Na+ at physiological ion level. To increase long-term stability of oligonucleotide in blood, the screened oligonucleotide was modified with an inverted thymine nucleotide whose 3′-terminus was connected to the 3′-terminus of the upstream nucleotide, acting as a blocking group to greatly improve antinuclease stability. Lastly, to avoid interference from background absorption and autofluorescence of blood, a G-quadruplex-binding, two-photon-excited ligand, EBMVC-B, was synthesized and chosen as the fluorescence reporter. Thus, based on selective K+ ion-induced formation of intermolecular G-quadruplex and EBMVC-B binding, this approach could linearly respond to K+ from 0.5 to 10 mM, which matches quite well with the physiologically relevant concentration of blood K+. Moreover, the system was highly selective for K+ against other metal ions, including Na+, Ca2+, Mg2+, Zn2+ common in blood. The practical application was demonstrated by direct detection of K+ from real blood samples by two-photon fluorescence technology. To the best of our knowledge, this is the first attempt to exploit molecular G-quadruplex-based fluorescent sensing for direct assay of blood target. As such, we expect that it will promote the design and practical application of similar DNA-based sensors in complex real systems. © 2016 American Chemical Society.
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英文
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A theranostic agent for invivo near-infrared imaging of β-amyloid species and inhibition of β-amyloid aggregation
作者：
Li, Yinhui;Xu, Di;Ho, See-Lok;Li, Hung-Wing* ;Yang, Ronghua* ;...
期刊：
Biomaterials ,2016年94:84-92 ISSN：0142-9612
通讯作者：
Li, Hung-Wing;Wong, Man Shing;Yang, Ronghua
作者机构：
[Wong, Man Shing; Ho, See-Lok; Li, Hung-Wing; Wong, MS; Xu, Di; Li, Yinhui] Hong Kong Baptist Univ, Dept Chem, Kowloon Tong, Peoples R China.;[Li, Yinhui] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Yang, Ronghua] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Li, HW; Wong, MS] H;[Yang, Ronghua] C;Hong Kong Baptist Univ, Dept Chem, Kowloon Tong, Peoples R China.;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.
关键词：
*Alzheimer's disease;*Beta-amyloid;*Inhibitor;*NIR imaging;*Theranostic agent
摘要：
Amyloid-β (Aβ) peptide as one of the main components of senile plaques is closely related to the onset and progression of incurable Alzheimer's disease (AD). Numerous efforts have been devoted to develop probes for Aβ species/plaque imaging for AD diagnostics and to develop aggregation inhibitors preventing formation of toxic soluble oligomeric Aβ for therapeutics. Herein, for the first time, a series of novel charged molecules, which can simultaneously perform near infra-red in vivo imaging of Aβ species/plaques in animal model and inhibition of self-aggregation of Aβ monomer from forming toxic oligomers, are reported. Among them, DBA-SLOH showed excellent blood-brain barrier (BBB) permeability and biocompatibility due to the incorporation of lipophilic alkyl chains with moderate length into the charged skeleton. Importantly, DBA-SLOH was found to have a high binding affinity toward Aβ species exhibiting a dramatic fluorescence enhancement upon interacting with Aβ species. Despite a weaker binding with Aβ monomers as compared to Aβ aggregates, DBA-SLOH could effectively prevent the Aβ1-40 and Aβ1-42 peptides from self-aggregation and forming toxic oligomers. This multifunctional fluorescent molecule shows promising potential as a theranostic agent for the diagnosis and therapy of AD. © 2016 Elsevier Ltd.
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英文
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In situ formation of fluorescent copper nanoparticles for ultrafast zero-background Cu2+ detection and its toxicides screening
作者：
Qing, Zhihe* ;Zhu, Lixuan;Yang, Sheng;Cao, Zhong;He, Xiaoxiao;...
期刊：
Biosensors and Bioelectronics ,2016年78:471-476 ISSN：0956-5663
通讯作者：
Qing, Zhihe;Yang, Ronghua
作者机构：
[Yang, Sheng; Yang, Ronghua; Qing, Zhihe; Zhu, Lixuan; Cao, Zhong] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.;[He, Xiaoxiao; Wang, Kemin] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.
通讯机构：
[Qing, ZH; Yang, RH] C;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.
关键词：
Copper ion;Detection;Fluorescence;Toxicide screening
摘要：
Copper pollution has become more and more serious in modern society as the increasing industrial emission and the acid mine drainage, and exposure to excess copper can result in damage to living organisms. Thus, the development of efficient strategy for copper ion (Cu2+) detection is very essential and significant. Here, a high-efficiency fluorescent method is proposed for Cu2+ monitoring. The detection mechanism is based on the in situ formation of fluorescent copper nanoparticles (CuNPs). When the water sample is polluted by Cu2+, fluorescence emission of CuNPs can be observed by a one-step manner, and the emission intensity is proportional to Cu2+ concentration. Attractively, besides its advantages in operation and good detection capability, the generation of fluorescent signal is ultrafast, with a good signal response in 1min; and there is no interference from background and other ions due to the in situ formation of signal unit. By virtue of its advantages, this strategy has been used to detect Cu2+ from polluted tap and river water samples, good performances demonstrate that the proposed method can be practically applied for Cu2+ monitoring in real drinking and environmental water. Simultaneously, great potential for Cu2+ toxicides screening has been verified by direct analysis of the effects of different model molecules on Cu2+, which will contribute to Cu2+-related sewage treatment and medical therapy. © 2015 Elsevier B.V.
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英文
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