Progress in biosensor based on DNA-templated copper nanoparticles
作者：
Qing, Zhihe* ;Bai, Ailing;Xing, Shuohui;Zou, Zhen;He, Xiaoxiao;...
期刊：
Biosensors and Bioelectronics ,2019年137:96-109 ISSN：0956-5663
通讯作者：
Qing, Zhihe;Yang, Ronghua
作者机构：
[Zou, Zhen; Bai, Ailing; Yang, Ronghua; Qing, Zhihe; Xing, Shuohui] Changsha Univ Sci & Technol, Sch Chem & Food Engn, Hunan Prov Engn Res Ctr Food Proc Aquat Biot Reso, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Changsha 410114, Hunan, Peoples R China.;[He, Xiaoxiao; Zou, Zhen; Yang, Ronghua; Wang, Kemin] Hunan Univ, Coll Chem & Chem Engn, Coll Biol Key Lab Bionanotechnol & Mol Engn Hunan, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.
通讯机构：
[Qing, ZH; Yang, RH] C;Changsha Univ Sci & Technol, Sch Chem & Food Engn, Hunan Prov Engn Res Ctr Food Proc Aquat Biot Reso, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Changsha 410114, Hunan, Peoples R China.
关键词：
Biosensor;Copper nanoparticles;DNA;Template
摘要：
During the last decades, by virtue of their unique physicochemical properties and potential application in microelectronics, biosensing and biomedicine, metal nanomaterials (MNs)have attracted great research interest and been highly developed. Deoxyribonucleic acid (DNA)is a particularly interesting ligand for templating bottom-up nanopreparation, by virtue of its excellent properties including nanosized geometry structure, programmable and artificial synthesis, DNA-metal ion interaction and powerful molecular recognition. DNA-templated copper nanoparticles (DNA-CuNPs)has been developed in recent years. Because of its advantages including simple and rapid preparation, high efficiency, MegaStokes shifting and low biological toxicity, DNA-CuNPs has been highly exploited for biochemical sensing from 2010, especially as a label-free detection manner, holding advantages in multiple analytical technologies including fluorescence, electrochemistry, surface plasmon resonance, inductively coupled plasma mass spectrometry and surface enhanced Raman spectroscopy. This review comprehensively tracks the preparation of DNA-CuNPs and its application in biosensing, and highlights the potential development and challenges regarding this field, aiming to promote the advance of this fertile research area. © 2019 Elsevier B.V.
语种：
英文
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印制电路板用化学镀金工艺的研究进展
作者：
吴道新;王毅玮;肖忠良;杨荣华;姚文娟
期刊：
材料保护 ,2019年52(6):108-115,178 ISSN：1001-1560
作者机构：
长沙理工大学化学与生物工程学院,湖南长沙,410014;[杨荣华; 肖忠良; 王毅玮; 吴道新; 姚文娟] 长沙理工大学
关键词：
印制电路板;化学镀金;无氰镀金;化学镀镍浸金;化学镀镍镀钯镀金
摘要：
化学镀金作为印制电路板表面处理工艺,能够提供可焊性、延展性和抗腐蚀性等良好的镀金层,因而被广泛关注和研究。综述了印刷电路板( PCB)上化学镀金的研究进展,介绍了化学镀金原理和化学镀金液组成,叙述了无氰镀金体系的发展,同时从PCB镍金和镍钯金工艺的角度,分析了镀镍层、镀钯层和镀金层之间的相互影响,并对置换镀金和还原镀金进行了讨论,指出后者在生产效率和镀层质量上具有较大优势,并将逐渐取代置换镀金工艺。
语种：
中文
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Highly Sensitive Detection of Bladder Cancer-Related miRNA in Urine Using Time-Gated Luminescent Biochip
作者：
Wang, Yingqian;Li, Zhihao;Lin, Qiaosong;Wei, Yurong;Wang, Jie;...
期刊：
ACS SENSORS ,2019年4(8):2124-2130 ISSN：2379-3694
通讯作者：
Yuan, Quan
作者机构：
[Wang, Yingqian; Yuan, Quan; Li, Zhihao; Wei, Yurong; Lin, Qiaosong; Li, Yingxue; Wang, Jie] Wuhan Univ, Coll Chem, Minist Educ, Key Lab Analyt Chem Biol & Med, Wuhan 430072, Hubei, Peoples R China.;[Yuan, Quan] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Mol Sci & Biomed Lab, Changsha 410082, Hunan, Peoples R China.;[Yang, Ronghua] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Yuan, Quan] W;[Yuan, Quan] H;Wuhan Univ, Coll Chem, Minist Educ, Key Lab Analyt Chem Biol & Med, Wuhan 430072, Hubei, Peoples R China.;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Mol Sci & Biomed Lab, Changsha 410082, Hunan, Peoples R China.
关键词：
long-lifetime luminescence;photonic crystals;miRNA detection;urine;autofluorescence
摘要：
Detection of biomarkers in complex samples is a significant health plan strategy for medical diagnosis, therapy monitoring, and health management. However, high background noise resulting from impurities and other analytes in complex samples has hampered the improvement of detection sensitivity and accuracy. Herein, an ultralow background biochip based on time-gated luminescent probes supported by photonic crystals (PCs) was successfully developed for detection of bladder cancer (BC)-related miRNA biomarkers with high sensitivity and specificity in urine samples. Coupled with the time-gated luminescence of long-lifetime luminescence probes and the luminescence-enhanced capability of PCs, the short-lived autofluorescence can be efficiently removed; thus, the detection sensitivity will be significantly improved. Benefiting from these merits, a detection limit of 26.3 fM is achieved. Furthermore, the biochip exhibits excellent performance in urinary miRNA detection, and good recoveries are also obtained. The developed biochip possesses unique properties of ultralow background and luminescence enhancement, thus offering a suitable tool for the detection of BC-related miRNA in urine. With rational design of probe sequences, the biochip holds great potential for many other biomarkers in real patient samples, making it valuable in areas such as medical diagnosis and disease evaluation. © 2019 American Chemical Society.
语种：
英文
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Hypoxia-triggered gene therapy: a new drug delivery system to utilize photodynamic-induced hypoxia for synergistic cancer therapy
作者：
Huang, Caixia;Zheng, Jing* ;Ma, Dandan;Liu, Na;Zhu, Cong;...
期刊：
JOURNAL OF MATERIALS CHEMISTRY B ,2018年6(40):6424-6430 ISSN：2050-750X
通讯作者：
Zheng, Jing
作者机构：
[Liu, Na; Huang, Caixia; Zheng, Jing; Zhu, Cong; Ma, Dandan; Li, Jishan] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Yang, Ronghua] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Zheng, Jing] H;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.
摘要：
The therapeutic effects of photodynamic therapy (PDT) are limited by cancer hypoxia because the PDT process is dependent on O 2 concentration. Based on this, a new living drug delivery system integrated PDT and hypoxia-triggered gene therapy is proposed, which is made up of three primary constituents: hypoxia-induced cleaved azobenzene (Azo) bridges, HIF-1α-against antisense oligonucleotide (ASO)/G4-constituted double-stranded DNA/RNA hybridization complex (DRHC) and the photosensitizer TMPyP4. During PDT, the continuous consumption of oxygen could remarkably facilitate an intracellular low-oxygen microenvironment. Then, the hypoxia-responsive Azo bridges were reduced by the highly expressed reductases to amines under the oxygen-deficient environment, resulting in a hypoxia-triggered ASO release and providing a synergistic therapy with PDT for suppression of tumor growth. This new drug delivery system opens a new avenue for the design and fabrication of smart drug delivery methods, which can deliver and release drugs according to the specific biological microenvironment in the body. © The Royal Society of Chemistry.
语种：
英文
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Misfolding of a DNAzyme for ultrahigh sodium selectivity over potassium
作者：
He, Yanping;Chen, Da* ;Huang, Po-Jung Jimmy;Zhou, Yibo;Ma, Lingzi;...
期刊：
Nucleic Acids Research ,2018年46(19):10262-10271 ISSN：0305-1048
通讯作者：
Chen, Da;Liu, Juewen
作者机构：
[He, Yanping; Chen, Da; Xu, Kexin] Univ Tianjin, State Key Lab Precis Measurement Technol & Instru, Tianjin 300072, Peoples R China.;[He, Yanping; Ma, Lingzi; Huang, Po-Jung Jimmy; Liu, Juewen] Univ Waterloo, Waterloo Inst Nanotechnol, Dept Chem, Waterloo, ON N2L 3G1, Canada.;[Yang, Ronghua; Zhou, Yibo] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410114, Hunan, Peoples R China.
通讯机构：
[Chen, Da; Liu, Juewen] U;Univ Tianjin, State Key Lab Precis Measurement Technol & Instru, Tianjin 300072, Peoples R China.;Univ Waterloo, Waterloo Inst Nanotechnol, Dept Chem, Waterloo, ON N2L 3G1, Canada.
关键词：
dna, catalytic;fluorescence;metals;cytokinesis;dna;thermodynamics;sodium
摘要：
Herein, the excellent Na+ selectivity of a few RNA-cleaving DNAzymes was exploited, where Na+ can be around 3000-fold more effective than K+ for promoting catalysis. By using a double mutant based on the Ce13d DNAzyme, and by lowering the temperature, increased 2-aminopurine (2AP) fluorescence was observed with addition of both Na+ and K+. The fluorescence increase was similar for these two metals at below 10 mM, after which K+ took a different pathway. Since 2AP probes its local base stacking environment, K+ can be considered to induce misfolding. Binding of both Na+ and K+ was specific, since single base mutations could fully inhibit 2AP fluorescence for both metals. The binding thermodynamics was measured by temperature-dependent experiments revealing enthalpy-driven binding for both metals and less coordination sites compared to G-quadruplex DNA. Cleavage activity assays indicated a moderate cleavage activity with 10 mM K+, while further increase of K+ inhibited the activity, also supporting its misfolding of the DNAzyme. For comparison, a G-quadruplex DNA was also studied using the same system, where Na+ and K+ led to the same final state with only around 8-fold difference in Kd. This study provides interesting insights into strategies for discriminating Na+ and K+.
语种：
英文
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Molecular Engineering of α-Substituted Acrylate Ester Template for Efficient Fluorescence Probe of Hydrogen Polysulfides
作者：
Guo, Jingru;Yang, Sheng* ;Guo, Chongchong;Zeng, Qinghai;Qing, Zhihe;...
期刊：
Analytical Chemistry ,2018年90(1):881-887 ISSN：0003-2700
通讯作者：
Yang, Sheng;Yang, Ronghua
作者机构：
[Yang, Sheng; Yang, Ronghua; Guo, Chongchong; Guo, Jingru; Qing, Zhihe; Cao, Zhong] Changsha Univ Sci & Technol, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Sch Chem & Biol Engn, Changsha 410114, Hunan, Peoples R China.;[Yang, Ronghua; Li, Jishan] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Zeng, Qinghai] Cent S Univ, Xiangya Hosp 3, Dept Dermatol, Changsha 410013, Hunan, Peoples R China.
通讯机构：
[Yang, S; Yang, RH] C;[Yang, Ronghua] H;Changsha Univ Sci & Technol, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Sch Chem & Biol Engn, Changsha 410114, Hunan, Peoples R China.;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.
摘要：
In this article, hydrogen polysulfide (H2Sn)-mediated Michael addition/cyclization cascade reactions toward acrylate ester analogues were exploited and utilized to construct novel and robust H2Sn-specific fluorescence probe for the first time. Through rational molecular engineering of the α-substituted acrylate ester template, the optimal candidate probe FP-CF3 containing trifluoromethyl-substituted acrylate ester group as recognition unit and 3-benzothiazol-7-hydroxycoumarin dye BHC as signal reporter can highly selectively detect H2Sn over other reactive sulfur species, especially biothiols including cysteine (Cys) and homocysteine (Hcy)/glutathione (GSH), with a rapid and significant turn-on fluorescence response (less than 60 s for response time and over 44-fold for signal-to-background ratio). The fast response and high selectivity of FP-CF3 for H2Sn could be attributed to a kinetically and spatially favored pentacyclic addition produced by the dual nucleophilic reaction of H2Sn with the CF3-substituted acrylate group. The big off-on fluorescence response is due to the pentacyclic intermediate results in the release of the highly fluorescent BHC. Moreover, it has been successfully applied in imaging of endogenous H2Sn fluctuation in living cells. © 2017 American Chemical Society.
语种：
英文
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Use of a small molecule as an initiator for interchain staudinger reaction: A new ATP sensing platform using product fluorescence
作者：
Yu, Huan;Zheng, Jing;Yang, Sheng;Asiri, Abdullah M.;Alamry, Khalid A.;...
期刊：
Talanta ,2018年178:282-286 ISSN：0039-9140
通讯作者：
Yang, Ronghua;Wang, Suhua
作者机构：
[Zhang, Kui; Yu, Huan; Sun, Mingtai] Chinese Acad Sci, Inst Intelligent Machines, Hefei 230031, Anhui, Peoples R China.;[Yu, Huan] Univ Sci & Technol China, Dept Mat Sci & Engn, Hefei 230026, Anhui, Peoples R China.;[Yang, Sheng; Zheng, Jing; Yang, Ronghua] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Changsha 410114, Hunan, Peoples R China.;[Alamry, Khalid A.; Asiri, Abdullah M.] King Abdulaziz Univ, NAAM Res Grp, Jeddah 21589, Saudi Arabia.;[Wang, Suhua] North China Elect Power Univ, Sch Environm & Chem Engn, Beijing 102206, Peoples R China.
通讯机构：
[Yang, Ronghua] C;[Wang, Suhua] N;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Changsha 410114, Hunan, Peoples R China.;North China Elect Power Univ, Sch Environm & Chem Engn, Beijing 102206, Peoples R China.
关键词：
Adenosine triphosphate;Fluorescence enhancement;Split aptamer;Staudinger reaction
摘要：
We demonstrated that a small molecule induced interchain Staudinger reaction can be employed for highly selective detection of adenosine triphosphate (ATP), an important energy-storage biomolecule. A designed ATP split aptamer (A1) was first functionalized with a weakly fluorescent coumarin derivative due to an azide group (azido-coumarin). The second DNA strand (A2) was covalently linked with triphenylphosphine, which could selectively and efficiently reduce azido to amino group through the Staudinger reaction. The A2 was then hybridized with a half of another designed longer DNA strand (T1). The second half of T1 was a split aptamer and selectively recognized ATP with A1 to form a sandwich structure. The specific interaction between ATP and the aptamers drew the two functionalized DNA strands (A1 and A2) together to initiate the interchain Staudinger reduction at fmol-nmol concentration level, hence produced fluorescent 7-aminocoumarin which could be used as an indicator for the presence of trace ATP. The reaction process had a concentration dependent manner with ATP in a large concentration range. Such a strategy of interchain Staudinger reaction can be extended to construct biosensors for other small functional molecules on the basis of judiciously designed aptamers.
语种：
英文
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A dsDNA-lighted fluorophore for monitoring protein-ligand interaction through binding-mediated DNA protection
作者：
Qing, Zhihe* ;Zhu, Lixuan;Hou, Lina;Zou, Zhen;Yang, Sheng;...
期刊：
中国科学：化学英文版 ,2018年61(12):1630-1636 ISSN：1674-7291
通讯作者：
Qing, Zhihe;Yang, Ronghua
作者机构：
[Yang, Sheng; Hou, Lina; Zou, Zhen; Yang, Ronghua; Qing, Zhihe; Zhu, Lixuan] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Hunan Prov Engn Res Ctr Food Proc Aquat Biot Reso, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Changsha grid4406699, Peoples R China.;[Yang, Sheng; Hou, Lina; Zou, Zhen; Yang, Ronghua; Qing, Zhihe; Zhu, Lixuan] Changsha Univ Sci & Technol, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Hunan Prov Engn Res Ctr Food Proc Aquat Biot Reso, Sch Chem & Biol Engn, Changsha 410114, Hunan, Peoples R China.
通讯机构：
[Qing, ZH; Yang, RH] C;Changsha Univ Sci & Technol, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Hunan Prov Engn Res Ctr Food Proc Aquat Biot Reso, Sch Chem & Biol Engn, Changsha 410114, Hunan, Peoples R China.
关键词：
dsDNA-lighted fluorophore;protein-ligand interaction;DNA protection;fluorescence detection
摘要：
Because of their important roles in cellular functions, life activities, drug screening, and disease treatment, the development of efficient methods for monitoring protein-ligand interaction is essential. In this study, inspired by our previous studies on DNA conformation-selective fluorescent indicators, we developed a new sensing platform for monitoring protein-ligand interaction and detecting protein activity based on binding-mediated DNA protection and the dsDNA-lighted fluorophore, ethyl-4-[3,6-bis (1-methyl-4-vinylpyridium iodine)-9H-carbazol-9-yl)] butanoate (EBCB). The ligand was purposefully linked to the 3ʹ-terminal of a hairpin DNA probe to selectively bind with the target protein and protect the DNA from cleavage by exonuclease III. By virtue of EBCB’s outstanding capacity to discriminate DNA conformation, the protein-ligand interaction could be effectively monitored through a fluorescence change in EBCB. A high fluorescence signal was detected when the hairpin DNA was protected in the presence of the target protein, whereas a much lower signal was observed in the presence of nontarget proteins. Our results demonstrated that the proposed strategy had high potential, such as high selectivity and relatively high sensitivity, for monitoring protein-ligand interaction and detecting protein activity. We believe these results will pave the way for applying dsDNA-lighted fluorophore EBCB as an effective signal transducer for DNA conformation transformation-mediated biochemical sensing. © 2018, Science China Press and Springer-Verlag GmbH Germany, part of Springer Nature.
语种：
英文
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Fluorescence detection of glutathione and oxidized glutathione in blood with a NIR-excitable cyanine probe
作者：
Liu, Chang-hui* ;Qi, Feng-pei;Wen, Fu-bin;Long, Li-ping;Liu, Ai-juan;...
期刊：
Methods and Applications in Fluorescence ,2018年6(2):024001 ISSN：2050-6120
通讯作者：
Liu, Chang-hui;Yang, Rong-hua
作者机构：
[Liu, CH; Yang, Rong-hua; Liu, Chang-hui] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.;[Liu, Chang-hui; Wen, Fu-bin; Qi, Feng-pei; Long, Li-ping] Hunan City Univ, Coll Mat & Chem Engn, Yiyang 413000, Peoples R China.;[Liu, CH; Yang, Rong-hua; Liu, Chang-hui] Beijing Univ Chinese Med, Sch Humanities, Beijing 100029, Peoples R China.
通讯机构：
[Liu, CH; Yang, RH] C;[Liu, Chang-hui] H;[Liu, CH; Yang, RH] B;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.;Hunan City Univ, Coll Mat & Chem Engn, Yiyang 413000, Peoples R China.
关键词：
near-infrared;probe;blood glutathione;GSSG;cyanine
摘要：
Cyanine has been widely utilized as a near infrared (NIR) fluorophore for detection of glutathione (GSH). However, the excitation of most of the reported cyanine-based probes was less than 800 nm, which inevitably induce biological background absorption and lower the sensitivity, limiting their use for detection of GSH in blood samples. To address this issue, here, a heptamethine cyanine probe (DNIR), with a NIR excitation wavelength at 804 nm and a NIR emission wavelength at 832 nm, is employed for the detection of GSH and its oxidized form (GSSG) in blood. The probe displays excellent selectivity for GSH over GSSG and other amino acids, and rapid response to GSH, in particular a good property for indirect detection of GSSG in the presence of enzyme glutathione reductase and the reducing agent nicotinamideadenine dinucleotide phosphate, without further separation prior to fluorescent measurement. To the best of our knowledge, this is the first attempt to explore NIR fluorescent approach for the simultaneous assay of GSH and GSSG in blood. As such, we expect that our fluorescence sensors with both NIR excitation and NIR emission make this strategy suitable for the application in complex physiological systems. © 2018 IOP Publishing Ltd.
语种：
英文
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Frontispiece: Selection and Screening of DNA Aptamers for Inorganic Nanomaterials
作者：
Dr. Yibo Zhou;Zhicheng Huang;Prof. Dr. Ronghua Yang;Prof. Dr. Juewen Liu*
期刊：
CHEMISTRY-A EUROPEAN JOURNAL ,2018年24(11):- ISSN：0947-6539
通讯作者：
Prof. Dr. Juewen Liu
作者机构：
[Dr. Yibo Zhou; Prof. Dr. Ronghua Yang] School of Chemistry and Biological Engineering, Changsha University of Science and Technology, Changsha, 410114 P. R. China;[Zhicheng Huang] Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario, N2L 3G1 Canada;[Prof. Dr. Juewen Liu] School of Chemistry and Biological Engineering, Changsha University of Science and Technology, Changsha, 410114 P. R. China<&wdkj&>Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario, N2L 3G1 Canada
通讯机构：
[Prof. Dr. Juewen Liu] S;School of Chemistry and Biological Engineering, Changsha University of Science and Technology, Changsha, 410114 P. R. China<&wdkj&>Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario, N2L 3G1 Canada
关键词：
adsorption;aptamers;DNA;graphene;nanomaterials
语种：
英文
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Selection and Screening of DNA Aptamers for Inorganic Nanomaterials
作者：
Zhou, Yibo;Huang, Zhicheng;Yang, Ronghua;Liu, Juewen*
期刊：
CHEMISTRY-A EUROPEAN JOURNAL ,2018年24(11):2525-2532 ISSN：0947-6539
通讯作者：
Liu, Juewen
作者机构：
[Yang, Ronghua; Zhou, Yibo; Liu, Juewen] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410114, Hunan, Peoples R China.;[Huang, Zhicheng; Liu, Juewen] Univ Waterloo, Waterloo Inst Nanotechnol, Dept Chem, Waterloo, ON N2L 3G1, Canada.
通讯机构：
[Liu, Juewen] C;[Liu, Juewen] U;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410114, Hunan, Peoples R China.;Univ Waterloo, Waterloo Inst Nanotechnol, Dept Chem, Waterloo, ON N2L 3G1, Canada.
关键词：
adsorption;aptamers;DNA;graphene;nanomaterials
摘要：
Searching for DNA sequences that can strongly and selectively bind to inorganic surfaces is a long-standing topic in bionanotechnology, analytical chemistry and biointerface research. This can be achieved either by aptamer selection starting with a very large library of ≈1014 random DNA sequences, or by careful screening of a much smaller library (usually from a few to a few hundred) with rationally designed sequences. Unlike typical molecular targets, inorganic surfaces often have quite strong DNA adsorption affinities due to polyvalent binding and even chemical interactions. This leads to a very high background binding making aptamer selection difficult. Screening, on the other hand, can be designed to compare relative binding affinities of different DNA sequences and could be more appropriate for inorganic surfaces. The resulting sequences have been used for DNA-directed assembly, sorting of carbon nanotubes, and DNA-controlled growth of inorganic nanomaterials. It was recently discovered that poly-cytosine (C) DNA can strongly bind to a diverse range of nanomaterials including nanocarbons (graphene oxide and carbon nanotubes), various metal oxides and transition-metal dichalcogenides. In this Concept article, we articulate the need for screening and potential artifacts associated with traditional aptamer selection methods for inorganic surfaces. Representative examples of application are discussed, and a few future research opportunities are proposed towards the end of this article. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
语种：
英文
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Visualizing Endogenous Sulfur Dioxide Derivatives in Febrile-Seizure-Induced Hippocampal Damage by a Two-Photon Energy Transfer Cassette
作者：
Yang, Sheng;Wen, Xidan;Yang, Xiaoguang;Li, Yuan;Guo, Chongchong;...
期刊：
Analytical Chemistry ,2018年90(24):14514-14520 ISSN：0003-2700
通讯作者：
Yang, Ronghua
作者机构：
[Wen, Xidan; Yang, Sheng; Yang, Xiaoguang; Guo, Chongchong; Yang, Ronghua; Zhou, Yibo; Li, Heping] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Changsha 410114, Hunan, Peoples R China.;[Li, Yuan] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.
通讯机构：
[Yang, Ronghua] C;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Changsha 410114, Hunan, Peoples R China.
摘要：
Febrile seizure (FS), a frequently encountered seizure disorder in pediatric populations, can cause hippocampus damage. It has been elucidated that sulfur dioxide (SO2) content is overproduced during the development of FS and related brain injury. Thus, monitoring in situ the level of endogenous SO2 in FS-related models is helpful to estimate the pathogenesis of FS-induced brain injury, but the effect detection method remains to be explored. Herein, we developed a two-photon energy transfer cassette based on an acedan-anthocyanidin scaffold, TP-Ratio-SO2, allowing us to achieve this purpose. TP-Ratio-SO2 specifically responds to SO2 derivatives (HSO3 -/SO3 2-) in an ultrafast fashion (less than 3 s), and HSO3 -/SO3 2- can be sensitively determined with a detection limit of 26 nM. Moreover, it exhibits significant changes in two well-resolved fluorescence emissions (Δ λ = 140 nm) by reacting with HSO3 -/SO3 2-, behaving as a ratiometric fluorescent sensor. Importantly, ratiometric imaging of endogenous SO2 derivatives generation in hyperpyretic U251 cells and in a rat model of FS-treated hippocampus damage was successfully carried out by TP-Ratio-SO2, demonstrating that it may be a promising tool for studying the role of SO2 in FS-associated neurological diseases. Copyright © 2018 American Chemical Society.
语种：
英文
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Azoreductase and Target Simultaneously Activated Fluorescent Monitoring for Cytochrome c Release under Hypoxia
作者：
Tang, Jianru;Huang, Caixia;Shu, Jinyong;Zheng, Jing* ;Ma, Dandan;...
期刊：
Analytical Chemistry ,2018年90(9):5865-5872 ISSN：0003-2700
通讯作者：
Zheng, Jing;Yang, Ronghua
作者机构：
[Huang, Caixia; Zheng, Jing; Tang, Jianru; Ma, Dandan; Li, Jishan] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Yang, Ronghua] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410076, Hunan, Peoples R China.;[Shu, Jinyong] First People Hosp Yueyang, Yueyang 414000, Peoples R China.
通讯机构：
[Zheng, Jing] H;[Yang, Ronghua] C;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410076, Hunan, Peoples R China.
摘要：
Hypoxia-induced cell apoptosis is closely related to degenerative diseases, autoimmune disorders, and tumor disease. In the process of apoptosis, the release of cytochrome c (Cyt c) is deemed to be a critical factor of the intrinsic pathway. Strategies for tracking Cyt c release in living cells based on the subcellular localization have been proposed recently. However, they are inherently lack of specificity for distinguishing the release of Cyt c in apoptotic process induced by hypoxia from other stimulus. In this paper, an azoreductase and target simultaneously activated fluorescent aptameric nanosensor integrating gold nanoparticles (AuNPs) and Cyt c-targeted aptamer-consisted double-stranded DNA hybridization complex (DSDHC) was proposed. It is worth noting that the employment of azobenzene moiety labeled on the DSDHC first ensured the aptameric nanosensor could be conjugated to the surface of AuNPs and then specifically reduced by hypoxia-related azoreductase. Upon Cyt c released from mitochondrion under hypoxia, the competitive displacement of Cyt c subsequently activated the fluorescence of the aptameric nanosensor and the fluorescence enhancement depended principally on the content of Cyt c release. Inspired by this, a new strategy for quantitative analysis and in situ imaging of Cyt c under hypoxic condition was proposed. The high spatial resolution monitoring of the dynamics of Cyt c release under hypoxia will offer a potentially rich opportunity to understand the apoptotic mechanism under hypoxic conditions, thus further facilitating risk assessment and risk reduction for hypoxic environments. © 2018 American Chemical Society.
语种：
英文
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"Trojan Horse" DNA Nanostructure for Personalized Theranostics: Can It Knock on the Door of Preclinical Practice?
作者：
Li, Yuan;Yang, Sheng;Zheng, Jing;Zou, Zhen;Yang, Ronghua* ;...
期刊：
LANGMUIR ,2018年34(49):15028-15044 ISSN：0743-7463
通讯作者：
Yang, Ronghua
作者机构：
[Li, Yuan; Zheng, Jing; Yang, Ronghua; Tan, Weihong] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Yang, Sheng; Zou, Zhen; Yang, Ronghua] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Yang, Ronghua] H;[Yang, Ronghua] C;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.
摘要：
Nanotheranostics, combing diagnostic and therapeutic components in an all-in-one nanomaterial, possess exciting potentials for precision nanomedicine. However, a major obstacle for current nanotheranostics to enter preclinical and/or clinical trials is the intrinsic toxicities of these nanomaterials. As an emerging biomaterial, the bioinspired DNA nanostructure shows advantages for constructing better nanotheranostics due to its excellent features, including native biocompatibility, full programmability, and ready accessibility. In this feature article, we highlight recent advances in the design of DNA-nanostructure-based diagnostics and/or therapeutics capable of specifically responding to biological stimuli in a dynamic way, with a particular focus on the design mechanism, responsive performance, and potential for preclinical and/or clinical trials in personalized theranostics.
语种：
英文
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Quantitative detection of exosomal microRNA extracted from human blood based on surface-enhanced Raman scattering
作者：
Ma, Dandan;Huang, Caixia;Zheng, Jing* ;Tang, Jianru;Li, Jishan;...
期刊：
Biosensors and Bioelectronics ,2018年101:167-173 ISSN：0956-5663
通讯作者：
Zheng, Jing;Yang, Ronghua
作者机构：
[Huang, Caixia; Zheng, Jing; Yang, Ronghua; Zheng, J; Tang, Jianru; Ma, Dandan; Li, Jishan] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Yang, Jinfeng] Cent S Univ, Xiangya Sch Med, Affiliated Canc Hosp, Hunan Canc Hosp, Changsha 410013, Hunan, Peoples R China.;[Yang, Ronghua] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Zheng, J; Yang, RH] H;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.
关键词：
Exosomal microRNA;Recycling amplification;Surface-enhanced Raman spectroscopy
摘要：
Since the nature of the exosomal lipid bilayer can allow miRNAs to be protected from degradation by cellular RNAses in body fluids, exosomal microRNA (miRNA) has become an ideal source of non-invasive biomarkers for the early diagnosis and prognosis. In this paper, a new surface-enhanced Raman scattering (SERS) analysis strategy combining stable SERS reporter element and duplex-specific nuclease (DSN)-assisted signal amplification for quantitative detection of exosomal miRNA extracted from human blood is proposed. Firstly, we prepared SERS signal reporter of Au@R6G@AgAu nanoparticles (R6G attachment on the gold nanoparticles, then encapsulated in AgAu alloy shell nanoparticles named as ARANPs) with an inter small nanogap to generate stable SERS signal. Then, ARANPs and separating substrate of silicon microbead (SiMB) were then covalently attached to the 3′- and 5′- end of capture probe (CP) targeting exosomal miRNA. Upon target miRNA binding, DNA in heteroduplexes could be specifically cleaved by DSN and resulted in the release of ARANPs from the surface of SiMB. Meanwhile, target miRNA remained intact and subsequently involved in the next round of target-recycling amplification. The combination of stable SERS intensity and signal amplification significantly improved the sensitivity of the sensing systems, resulting in detection limits of 5 fM. More importantly, this method also could be used for the detection of exosomal miRNAs extracted from the blood collected from patients of recurrence in non-small-cell lung cancer (NSCLC), with a detection of 5.0 μL of sample volume, which has potential for point-of-care testing (POCT) in clinical analysis. ©2017 Elsevier B.V.
语种：
英文
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A Ratiometric Two-Photon Fluorescent Cysteine Probe with Well-Resolved Dual Emissions Based on Intramolecular Charge Transfer-Mediated Two-Photon-FRET Integration Mechanism
作者：
Yang, Sheng;Guo, Chongchong;Li, Yuan;Guo, Jingru;Xiao, Jie;...
期刊：
ACS SENSORS ,2018年3(11):2415-2422 ISSN：2379-3694
通讯作者：
Yang, Ronghua
作者机构：
[Xiao, Jie; Yang, Sheng; Guo, Chongchong; Guo, Jingru; Li, Jiangsheng; Yang, Ronghua; Qing, Zhihe] Changsha Univ Sci & Technol, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Sch Chem & Biol Engn, Changsha 410114, Hunan, Peoples R China.;[Li, Yuan; Yang, Ronghua] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Li, Yuan; Yang, Ronghua] Hunan Univ, Collaborat Innovat Ctr Chem & Mol Med, Changsha 410082, Hunan, Peoples R China.
通讯机构：
[Yang, Ronghua] C;[Yang, Ronghua] H;Changsha Univ Sci & Technol, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Sch Chem & Biol Engn, Changsha 410114, Hunan, Peoples R China.;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;Hunan Univ, Collaborat Innovat Ctr Chem & Mol Med, Changsha 410082, Hunan, Peoples R China.
关键词：
two-photon;FRET;ICT;ratiometric fluorescent probe;cysteine;bioimaging
摘要：
The development of an efficient ratiometric two-photon fluorescence imaging probe is crucial for in situ monitoring of biothiol cysteine (Cys) in biosystems, but the current reported intramolecular charge transfer (ICT)-based one suffers from serious overlap between the shifted emission bands. To address this issue, we herein for the first time constructed an ICT-mediated two-photon excited fluorescence resonance energy transfer (TP-FRET) system consisting of a two-photon fluorogen benzo[h]chromene and a Cys-responsive benzoxadiazole-analogue dye. Different from a previous mechanism that utilized single two-photon fluorogen to acquire a ratiometric signal, ICT was used to switch on the TP-FRET process of the energy transfer dyad by eliciting an absorption shift of benzoxadiazole with Cys to modulate the spectral overlap level between benzo[h]chromene emission and benzoxadiazole absorption, resulting in two well-separated emission signal changes with large emission wavelength shift (120 nm), fixed two-photon excitation maximum (750 nm), and significant variation in fluorescence ratio (over 36-fold). Therefore, it can be successfully employed to ratiometrically visualize Cys in HeLa cells and liver tissues. Importantly, this new ICT-mediated TP-FRET integration mechanism would be convenient for designing ratiometric two-photon fluorescent probes with two well-resolved emission spectra suitable for high resolution two-photon fluorescence bioimaging. © 2018 American Chemical Society.
语种：
英文
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Ratiometric Visualization of NO/H2S Cross-Talk in Living Cells and Tissues Using a Nitroxyl-Responsive Two-Photon Fluorescence Probe
作者：
Zhou, Yibo;Zhang, Xiufang;Yang, Sheng* ;Li, Yuan;Qing, Zhihe;...
期刊：
Analytical Chemistry ,2017年89(8):4587-4594 ISSN：0003-2700
通讯作者：
Yang, Ronghua;Yang, Sheng
作者机构：
[Li, Yuan; Zhang, Xiufang; Zheng, Jing; Yang, Ronghua; Zhou, Yibo; Li, Jishan] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Li, Yuan; Zhang, Xiufang; Zheng, Jing; Yang, Ronghua; Zhou, Yibo; Li, Jishan] Hunan Univ, Collaborat Innovat Ctr Chem & Mol Med, Changsha 410082, Hunan, Peoples R China.;[Yang, Sheng; Yang, Ronghua; Qing, Zhihe] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Changsha 410114, Hunan, Peoples R China.
通讯机构：
[Yang, Ronghua] H;[Yang, S; Yang, RH] C;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;Hunan Univ, Collaborat Innovat Ctr Chem & Mol Med, Changsha 410082, Hunan, Peoples R China.;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Changsha 410114, Hunan, Peoples R China.
摘要：
It is of scientific significance to explore the intricate relationship between two crucial gasotransmitters nitric oxide (NO) and hydrogen sulfide (H2S) because they exert similar and interdependent biological actions within the living organisms. Nevertheless, visualization of the NO/H2S crosstalk using effective molecular imaging tools remains challenging. To address this issue, and given that nitroxyl (HNO) has been implicated as the interdependent production of NO and H2S via a network of cascading chemical reactions, we herein design a ratiometric two-photon fluorescent probe for HNO, termed TP-Rho-HNO, which consists of benzo[h]chromene-rhodol scaffold as two-photon energy transfer cassette with phosphine moiety as specific HNO recognition unit. The newly proposed probe has been successfully applied in ratiometric two-photon bioimaging of endogenous HNO derived from NO and H2S interaction in the human umbilical vein cells (HUVECs) and as well as in rat brain tissues. Intriguingly, the imaging results consistently demonstrate that the mutually dependent upgeneration of H2S and NO are present in living biosystems, indicating that this molecular probe would provide a powerful approach to elucidate the chemical foundation for the anfractuous cross-talk between the NO and H2S signaling pathways in biology. © 2017 American Chemical Society.
语种：
英文
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Programmable DNA triple-helix molecular switch in biosensing applications: from in homogenous solutions to in living cells
作者：
Tang, Pinting;Zheng, Jing* ;Tang, Jianru;Ma, Dandan;Xu, Weijian;...
期刊：
Chemical Communications ,2017年53(16):2507-2510 ISSN：1359-7345
通讯作者：
Zheng, Jing;Yang, Ronghua
作者机构：
[Xu, Weijian; Tang, Pinting; Zheng, Jing; Yang, Ronghua; Zheng, J; Tang, Jianru; Ma, Dandan; Li, Jishan] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Yang, Ronghua; Cao, Zhong] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Changsha 410114, Hunan, Peoples R China.
通讯机构：
[Zheng, J; Yang, RH] H;[Yang, Ronghua] C;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Changsha 410114, Hunan, Peoples R China.
摘要：
Herein, we demonstrated a new gold nanoparticles (AuNPs)-integrated programmable triple-helix molecular switch (THMS) to realize the biosensing of multiple targets from in homogenous solution to in living cells. The results demonstrated that this proposed programmable THMS could be successfully used for imaging multiple messenger RNA (mRNA) in living cells and it significantly extends the scope of the THMS sensing platform.
语种：
英文
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Technologies for analysis of circulating tumour DNA: Progress and promise
作者：
Zou, Zhen;Qi, Peng;Qing, Zhihe;Zheng, Jing;Yang, Sheng;...
期刊：
TRAC-TRENDS IN ANALYTICAL CHEMISTRY ,2017年97:36-49 ISSN：0165-9936
通讯作者：
Yang, Ronghua
作者机构：
[Qi, Peng; Yang, Sheng; Zou, Zhen; Yang, Ronghua; Qing, Zhihe; Chen, Weiju] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.;[Yang, Ronghua; Zheng, Jing] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.
通讯机构：
[Yang, Ronghua] C;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.
关键词：
Circulating tumour DNA;Epigenetic changes;Point mutations;Polymerase chain reaction;Sequencing
摘要：
Circulating tumour DNA (ctDNA) isolated from peripheral blood has recently been shown to be a biomarker to detect gene mutations for the diagnosis, treatment, and prognosis of cancer. Utilizing ctDNA as the liquid biopsy has significant potential to pave the way toward a better understanding of cancer at the molecular level and improve patient outcomes in the future. Over the past decades, a large number of efforts have been devoted to the development of valid methods for analysing ctDNA, which provide researchers and clinicians a variety of tools to detect and monitor tumours. In this review, we displayed an overview of current representative technologies for the detection of ctDNA and discuss recent technical advancements. Then, the challenges and outlooks in this promising field are featured on the basis of its current development. © 2017
语种：
英文
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近红外双发射荧光探针比率检测高/半胱氨酸
作者：
刘长辉;李银辉;齐风佩;龙立平;杨荣华
期刊：
中国科学：化学 ,2017年47(8):1015-1021 ISSN：1674-7224
作者机构：
长沙理工大学化学与生物学院, 黑茶金花湖南省重点实验室, 长沙, 410004;湘潭大学化学学院, 湘潭, 411105;湖南城市学院化学与环境工程学院, 黑茶金花湖南省重点实验室, 益阳, 413000;长沙理工大学化学与生物学院, 长沙, 410004;[刘长辉] 长沙理工大学化学与生物学院, 黑茶金花湖南省重点实验室, 长沙, 410004
关键词：
高/半胱氨酸;近红外;双发射;比率检测;荧光探针
摘要：
合成的吲哚菁染料Cy5-A对高/半胱氨酸展现显著的荧光响应.高/半胱氨酸浓度的增加引发其荧光强度在660 nm处减弱,相应地在700 nm处增强,且700 nm与660 nm处的比率荧光变化与高胱氨酸(40.0~200.0 μM)及半胱氨酸(10.0~100.0 μM)浓度呈现良好的线性关系,最低检测限分别为13.2 μM与2.67 μM.通过核磁共振谱和质谱研究了Cy5-A与高/半胱氨酸的作用机制.研究表明, Cy5-A中的醛基与高/半胱氨酸发生环合反应,从而诱导产生近红外双发射光谱,探针响应不受其他干扰物的影响.因此, Cy5-A是一种简单高效的反应型荧光探针,可成功应用于血清样品中高/半胱氨酸的准确检测.
语种：
中文
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