摘要：
The purpose of this work was to indirect label IgG with fac-[188Re(CO)3(H2O)3]+ and to check the radiochemical behavior of the labeled product. The compound of (bis(2-pyridylmethyl)-amino)-acetic acid (L2H) was synthesized and labeled with fac-[188Re(CO)3(H2O)3]+. The labeling yield of 188Re(CO)3–L2H was more than 90%. The effects of protein concentration, reaction time, pH and reaction temperature of labeling of IgG with 188Re(CO)3–L2H were investigated. The conjugation conditions were optimized. The labeled product was analyzed by size exclusion HPLC and TLC. The stability of 188Re(CO)3–L2H–IgG in vitro was high. The results of this study may be useful for [188Re(CO)3(H2O)3]+ labeling of protein for radioimmunotherapy.
摘要：
The effect of mechanical alloying on Zn-Sb alloy system is investigated with X-ray diffraction (XRD), laser grain size analysis and differential scanning calorimetry (DSC) respectively. The results of laser particle size analysis shows that the particle size decreases with increasing of the grinding time between 0 and 24 h. XRD and DSC results indicate that longer the grinding time of Zn-Sb is, the more content of Zn4Sb3 become in the product in this process.
摘要：
A novel optical biosensor with gold surface bound silver nanoparticles has been constructed for detection of bio-molecules like antigen, antibody protein and DNA plasmid, which exhibited distinct optical properties of localized surface plasmon resonance (LSPR). The silver colloidal nanoparticles were prepared by using sodium borohydride reduction of silver nitrate and characterized by using UV-vis absorption spectroscopy, transmission electron microscopy (TEM), and cyclic voltammetry (CV), indicating that the silver colloidals were pure, sphere-shaped and narrow-dispersed size distribution with a diameter of 10 plusmn 0.5 nm. The silver nanoparticles were well assembled on the gold surface. It caused 3.08 nm of red shift in the peak wavelength (lambdamax) of LSPR spectrum for the nanosensor exposed to c-Myc (3C7) mouse monoclonal IgG and 3.36 nm of red shift to the corresponding antibody by the immuno-reaction subsequently. The LSPR nanobiosensor has been successfully applied to recognition of a DNA plasmid of pCMV-Myc Mammalian Expression Vector.
摘要：
The effects of multi-walled carbon nanotubes (MWNTs) on the phase change enthalpy (Delta H) and the thermal conductivity (kappa) of a solid-liquid phase change materials (PCM), palmitic acid (PA), have been investigated. The results showed that both the Delta H and the kappa of the composite were lower than that of PA when the loading of MWNTs was small. As the concentration of MWNTs in the composites increased, the Delta H of the composites was slightly improved and then decreased linearly. However, the kappa of the composites was monotonously increased from the minimum value. When the loading of MWNTs increased to 5% and no surfactant was added, the kappa of the composite was enhanced to be 26% higher than that of PA. The kappa of the composite could be enhanced by CTAB instead of SDBS when the loading of MWNTs was small, as SDBS showed no obvious effect on the kappa of the composites. Furthermore, the effects of surface modification of MWNTs on the Delta H and the kappa of the composites have also been investigated.
摘要：
We have proposed an electrochemical detection method for DNA molecules based on an extended gate field effect transistor (EGFET) sensing chip which consists of one gold plate for molecule recognition and FET part for signal transduction. DNA Probes were immobilized on the gold plate by forming mixed monolayers of thiolated single-stranded oligonucleotide (HS-ssDNA) and alkanethiols, like 6-hydroxy-1-hexanethiol (6-HHT). Electrode reaction corresponding to the reductive desorption of the adsorbed monolayers in strong alkali solution was presented for quantification of surface density of DNA by using fast cyclic voltammetry (FCV). The passivation effects of surface modification with different functional groups on the potential behavior of the gold electrode were also examined. It was feasible to use EGFET chip for detection of DNA hybridization reaction, that the hybridization efficient was estimated to be about 40%.
摘要：
A microbial fuel cell (MFC) was constructed using polypyrrole (PPy) coated carbon nanotubes (CNTs) composite as an anode material and Escherichia coli as the biocatalyst. The composite PPy-CNTs were synthesized by the in situ chemical polymerization of pyrrole on the CNTs using ammonium persulfate as an oxidant. The electrocatalytic behaviors of the composite modified anode were investigated by means of cyclic voltammetry, electrochemical impedance spectroscopy and discharge experiments. The PPy-CNTs modified anode showed better electrochemical performance than that of plain carbon paper. The amount of the loading of the composite on the anode was also investigated. The power output of the MFC increased along with the increase of the composite loading. In the absence of exogenous electron mediators, the MFC with the composite modified anode contained 5 mg cm−2 PPy-CNTs exhibited a maximum power density 228 mW m−2, which is much higher than those reported in the literature so far for E. coli using efficient electron mediators. These results show that the PPy-CNTs composite anode is promising for MFC application.
摘要：
An optical biosensor with surface bound gold cap-shaped nano particles like polystyrene nanospheres has been constructed for detection of bio-molecules like antigen, antibody protein and DNA plasmid, which exhibited distinct optical properties of localized surface plasmon resonance (LSPR). It caused 1.09 nm red shift in the peak wavelength (lambda<sub>max</sub>) of LSPR spectrum for the nanosensor exposed to c-Myc (3C7) mouse monoclonal IgG and 3.53 nm red shift to the corresponding antibody by the immuno -reaction subsequently. The kinetic process for the activity of the c-Myc (3C7) antigen lost with the time has been examined. The LSPR nanobiosensor was also applied to recognition of a DNA plasmid of pCMV-Myc Mammalian Expression Vector, which would provide valuable information on molecular biology in clinical medicine, disease prediction, gene mutation and gene diagnosis.
摘要：
The properties of resveratrol (3′, 4′, 5-trihydroxystlbene, RST) were for the first time evaluated as a potential substrate for horseradish peroxidase (HRP)-catalyzed fluorogenic reaction. The properties of RST for use as fluorogenic substrates for HRP and its application in immunoassays were compared with commercially available substrates such as p-hydroxyphenylpropionic acid (pHPPA), chavicol and Amplex red by a fluoroimmunosensing method in the use of Schistosomia japonicum antibody (SjAb) as a model analyte. The fluoroimmunosensing device was constructed by dispersing Schistosomia japonicum antigen (SjAg), nano-Ag/SiO2 particles and sol-gel at low temperature. In pH 5.8 Britton-Robinson buffer (B-R), HRP-SjAb conjugates can catalyze the polymerization reaction of RST by H2O2 forming fluorescent dimmers. The increase of the fluorescence intensity of the dimmers product at emission of 462 nm (excitation: 315 nm) is proportional to the concentration of HRP-SjAb binding to the SjAg entrapped in the nano-Ag/SiO2 particles-sol-gel matrix. A competitive binding assay has been used to determine SjAb in rabbit serum with the aid of SjAb labeled with HRP. Substrate RST showed comparable ability for HRP detection and its enzyme-linked immunosensing reaction system, in a linear detection ranging of 1.5×10−6–7.3×10−4 g/L and with a detection limit of 1.5×10−6 g/L. The immobilized biocomposites surface could be regenerated by simply polishing with an alumina paper, with an excellent reproducibility (RSD = 4.7%). The proposed method has been successfully used for analysis of the rabbit serum sample with satisfactory results.
作者机构：
[陈平; 张玲; 崔正丹; 曹忠; 盛智勇] School of Chemistry and Environmental Engineering, Changsha University of Science and Technology, Changsha 410076, China;[徐芬; 孙立贤] Materials and Thermochemistry Laboratory, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
通讯机构：
[Zhang, L.] S;School of Chemistry and Environmental Engineering, Changsha University of Science and Technology, China
作者机构：
[Sun, Li-Xian; Zhang, Jian; Liu, Ying-Ya; Xu, Fen; Zhang, Tao] Chinese Acad Sci, Dalian Inst Chem Phys, Mat & Thermochem Lab, Dalian 16023, Peoples R China.;[Zhang, Jian; Liu, Ying-Ya] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China.;[Zhao, Yi; You, Wan-Sheng] Liaoning Normal Univ, Inst Chem Functionalized Mat, Fac Chem & Chem Engn, Dalian 116029, Peoples R China.;[Yang, Daowu; Zeng, Julan; Cao, Zhong] Changsha Univ Sci & Technol, Sch Chem & Environm Engn, Changsha 410076, Peoples R China.
通讯机构：
[Sun, Li-Xian] C;Chinese Acad Sci, Dalian Inst Chem Phys, Mat & Thermochem Lab, Dalian 16023, Peoples R China.
摘要：
A lithium-based coordination polymer is synthesized from 1,3-benzene dicarboxylate acid with lithium perchlorate through a solvothermal way. The complex features a 3D hydrophilic structure. The adsorption of water and organic solvents on this coordination polymer was investigated in situ by quartz crystal microbalance (QCM), which indicated that this framework is highly sensitive to water and methanol.
