摘要：
Background: Increased occurrence of chronic syndromes has prompted researchers to investigate and develop drugs and methods for controlling chronic syndromes with a view to improve human health and reduce early aging. Material and methods: Human trials: After the allotted multivitamin pills or placebo pills had been taken for a stipulated period of about 2 months, the volunteers filled out feedback forms on curative effects of the pills in line with the health examination reports. The effects of the multivitamin on various symptoms or diseases and dysfunctions of the chronic metabolic syndromes were noted and evaluated based on the information provided in forms. Animal experiments: Mouse aging model induced by D-galactose were administered the multivitamin by oral gavage every morning. At the end of the sixth week, activity or content of the components associated with ageing and anti-aging in the brain and liver of the aging mice were determined to investigate the mechanisms of the new multivitamin on chronic metabolic syndromes and aging. Results: We found that multivitamin can eliminate or attenuate 38 types of symptoms or dysfunctions of the investigated metabolic syndromes; and that it has both preventive and curative/adjunctive therapeutic effects on the metabolic syndromes. The effects of this multivitamin on components associated with aging and anti-aging were significantly decreased - malondialdehyde content and monoamine oxidase activity but significantly increased activity of superoxide dismutase and glutathione peroxidase. This multivitamin has significant anti-aging effects. Conclusion: Supplementing with this multivitamin can prevent and provide treatment/adjunctive therapy for these chronic metabolic syndromes and delay the aging process.
通讯机构：
Jiangsu Key Laboratory of Atmospheric Environmental Monitoring and Pollution Control High-tech Research, Nanjing University of Information Science and Technology, Nanjing, China
摘要：
Herein, we proposed a new electrochemical sensing strategy for T2DM-related SNP detection via DNA-mediated growth of AgNPs on a SWCNT-modified electrode. Coupled with RNase HII enzyme assisted amplification, this approach could realize T2DM-related SNP assay and be applied in crude extracts of carcinoma pancreatic beta-cell lines.
摘要：
Sclerotinia disease, caused by Sclerotinia sclerotiorum, is one of the most serious plant diseases in China. Research on the mechanism of disease resistance to S. sclerotiorum will help solve control problems. In this study, near-isogenic lines were first used in combination with the proteomic technique. A comparison of protein expression profiles in a susceptible line with those in a resistant line during the interaction of adult Brassica napus with S. sclerotiorum resulted in the identification of 20 important proteins related to disease resistance. Those proteins were then determined to be involved in various functions, including pathogen resistance, antioxidation, and transcription regulation. Our finding showed that some proteins involved in defence-a glycine rich protein (GRP); a trypsin inhibitor protein (TIP); two heat shock proteins (HSPs); and a thiol methyltransferase (TMT)-were upregulated or expressed specially in the resistant B. napus lines. These proteins contribute to ROS (reactive oxygen species) elimination and pathogen-defence in the resistant line, which would help the host defend itself against S. sclerotiorum. As a consequence, the onset of PCD (programmed cell death) was delayed, and the spread of S. sclerotiorum was slowed in the resistant line. Presented results underline the role of specific proteins in the disease process. By building on these results, future research may help determine the genes that are important in conveying resistance to S. sclerotiorum in B. napus.
摘要：
A newly created multivitamin possesses many protective health functions. To investigate its safety when applied in medical treatment and when used as a food supplement, we studied its acute oral toxicity and 13-week oral toxicity in mice. The results showed that the oral lethal dose, 50% (LD50) of the biomass of the multivitamin in mice was greater than 2492 mg/kg body weight (BW) and that poisoned mice recovered within 72 h. The no observed effect level (NOEL) of long-term consumption was more than 249.3 mg/kg BW for haematological parameters, clinical chemistry parameters, histopathological examination of organs, food consumption, BW, ratio of organ weight to BW and other physiological parameters and conditions. Therefore, we conclude that dosages of up to 249.3 mg/kg BW/day of this multivitamin do not cause chronic toxicity in animals. Administration of this multivitamin may even improve the resistance of animals to negative environmental factors and may be safe for long-term consumption to enhance the health of individuals in accordance with the prescribed dosage (1.4 similar to 4.2 mg/kg BW/day). (C) 2012 Elsevier Ltd. All rights reserved.
关键词：
Chemical fingerprint;Chromatographic classification;Gastrodia tuber;Gastrodin content;HPLC;Quality assessment
摘要：
The Gastrodia tuber and its active component, gastrodin, have many pharmacological effects. In this study, optimized high performance liquid chromatography (HPLC) parameters were employed to determine the chemical fingerprints and gastrodin content of nine Gastrodia tuber populations. Based on the degree of similarity of the chemical fingerprints, the nine Gastrodia tuber populations were grouped into one of the three different classes. Class I Gastrodia tubers had the highest content of gastrodin and were thus, regarded as possessing the highest quality. Of the class I Gastrodia tuber samples, those from Yichang, Hubei and Shimen, Hunan, were identified as the “best.” Close relationships were detected among the chemical fingerprints, gastrodin content and place of origin of the Gastrodia tubers. Hence, these findings may be applied in assessing the quality of Gastrodia tubers and in identifying and segregating poor quality Gastrodia populations from those of good quality. Key words: Chemical fingerprint, chromatographic classification, Gastrodia tuber, gastrodin content, HPLC, quality assessment.
作者：
Xiao-Shun Shu;Jin-Hai Lv;Jun Tao;Guo-Ming Li;Ning Mae
期刊：
Medicinal Plants - International Journal of Phytomedicines and Related Industries,2010年2(2):161-164 ISSN：0975-4261
通讯作者：
Shu, X.-S.
作者机构：
[Shu X.-S.; Tao J.; Li G.-M.] Department of Biology, College of Chemistry and Biology, ChangSha University of Science and Technology, ChangSha 410 076, Hunan, China;[Lv J.-H.] Department of Biology, Huaihua College, HuaiHua 418 000, Hunan, China;[Mae N.] Changsha Medical College, Changsha 410 219, China
通讯机构：
Department of Biology, College of Chemistry and Biology, ChangSha University of Science and Technology, China
摘要：
The ethyl acetate extract, aqueous extract and membrane separation extract from the tubers of Smilax china were investigated for anti-bacterial and anti-fungal activity by agar plate disc-diffusion assay. All the three extracts showed anti-bacterial effects against all Gram-positive bacteria, Gram-negative bacteria and fungi tested. Among anti-bacterial activities of these extract, the ethyl acetate extracts exhibited stronger anti-microbial activity against Gram-positive bacteria as compared to aqueous extract and membrane separation extract showed similar to and even stronger anti-microbial activity than the aqueous extract, which may be that the former could possess higher concentration of the flavonoids and membrane separation extract possess higher concentration of the effective ingredients that prove that it is feasible that aqueous extract was further purified by membrane separation technique.
会议名称：
The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议)
会议时间：
2009-06-11
会议地点：
北京
会议论文集名称：
The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议)论文集
关键词：
Bioinformatics;DNA cloning;DNA fingerprint;DNA marker sequence;Gastrodia tuber;Genomic DNA sequence
摘要：
Gastrodia tuber has many pharmacological effects. DNA fingerprints of Gastrodia tuber were determined using randomly amplified polymorphic DNA (RAPD) technique, of which the desired DNA segments were selected and recovered. Positive DNA clones were obtained through ligation techniques, transformation, blue/white color screenings, cloning and identifying. Novelty of the cloned DNA and its potential importance were confirmed by sequencing DNA and bioinformatics analyzing. The results showed the DNA sequences were proven to be a new discovery, Sequence 1 and 3 had 3 and 1 Open Reading Frames (ORF), respectively. DNA sequence 2 have 13 ORFs, which can express 8 unintegrated function domains. The DNA sequence deserve further researching. The distribution of these 3 DNA sequences varied greatly among the populations, whereby DNA sequence 1 exist only in the populations that show the highest content of effective component gastrodin. The findings may be applied in exploiting genomic DNA and genetic classification of Gastrodia tuber populations.
关键词：
DNA polymorphism;Gastrodia elata Blume;Gastrodin;Genetic relationships;Chemical fingerprints
摘要：
Gastrodia tuber and its component gastrodin have many pharmacological effects. The chemical fingerprints and gastrodin contents of eight Gastrodia populations were determined, and the genomic DNA polymorphism of the populations was investigated. Genetic distance coefficients among the populations were calculated using the DNA polymorphism data. A dendrogram of the genetic similarities between the populations was constructed using the genetic distance coefficients. The results indicated that the genomic DNA of Gastrodia tubers was highly polymorphic; the eight populations clustered into three major groups, and the gastrodin content varied greatly among these groups. There were obvious correlations among genetic makeup, gastrodin content, and place of origin. The ecological environments in Guizhou and Shanxi may be conducive to evolution and to gastrodin biosynthesis, and more suitable for cultivation of Gastrodia tubers. These findings may provide a scientific basis for overall genetic resource management and for the selection of locations for cultivating Gastrodia tubers.
