摘要：
Background: Increased occurrence of chronic syndromes has prompted researchers to investigate and develop drugs and methods for controlling chronic syndromes with a view to improve human health and reduce early aging. Material and methods: Human trials: After the allotted multivitamin pills or placebo pills had been taken for a stipulated period of about 2 months, the volunteers filled out feedback forms on curative effects of the pills in line with the health examination reports. The effects of the multivitamin on various symptoms or diseases and dysfunctions of the chronic metabolic syndromes were noted and evaluated based on the information provided in forms. Animal experiments: Mouse aging model induced by D-galactose were administered the multivitamin by oral gavage every morning. At the end of the sixth week, activity or content of the components associated with ageing and anti-aging in the brain and liver of the aging mice were determined to investigate the mechanisms of the new multivitamin on chronic metabolic syndromes and aging. Results: We found that multivitamin can eliminate or attenuate 38 types of symptoms or dysfunctions of the investigated metabolic syndromes; and that it has both preventive and curative/adjunctive therapeutic effects on the metabolic syndromes. The effects of this multivitamin on components associated with aging and anti-aging were significantly decreased - malondialdehyde content and monoamine oxidase activity but significantly increased activity of superoxide dismutase and glutathione peroxidase. This multivitamin has significant anti-aging effects. Conclusion: Supplementing with this multivitamin can prevent and provide treatment/adjunctive therapy for these chronic metabolic syndromes and delay the aging process.
通讯机构：
Jiangsu Key Laboratory of Atmospheric Environmental Monitoring and Pollution Control High-tech Research, Nanjing University of Information Science and Technology, Nanjing, China
摘要：
Herein, we proposed a new electrochemical sensing strategy for T2DM-related SNP detection via DNA-mediated growth of AgNPs on a SWCNT-modified electrode. Coupled with RNase HII enzyme assisted amplification, this approach could realize T2DM-related SNP assay and be applied in crude extracts of carcinoma pancreatic beta-cell lines.
关键词：
Chemical fingerprint;chromatographic classification;Gastrodia tuber;gastrodin content;HPLC;quality assessment.
摘要：
The Gastrodia tuber and its active component, gastrodin, have many pharmacological effects. In this study, optimized high performance liquid chromatography (HPLC) parameters were employed to determine the chemical fingerprints and gastrodin content of nine Gastrodia tuber populations. Based on the degree of similarity of the chemical fingerprints, the nine Gastrodia tuber populations were grouped into one of the three different classes. Class I Gastrodia tubers had the highest content of gastrodin and were thus, regarded as possessing the highest quality. Of the class I Gastrodia tuber samples, those from Yichang, Hubei and Shimen, Hunan, were identified as the “best.” Close relationships were detected among the chemical fingerprints, gastrodin content and place of origin of the Gastrodia tubers. Hence, these findings may be applied in assessing the quality of Gastrodia tubers and in identifying and segregating poor quality Gastrodia populations from those of good quality. Key words: Chemical fingerprint, chromatographic classification, Gastrodia tuber, gastrodin content, HPLC, quality assessment.
作者：
Shu Xiao-Shun;Lv Jin-Hai;Tao Jun;Li Guo-Ming;Mae Ning
期刊：
Medicinal Plants - International Journal of Phytomedicines and Related Industries,2010年2(2):161-164 ISSN：0975-4261
通讯作者：
Shu, X.-S.
作者机构：
Department of Biology, College of Chemistry and Biology ChangSha University of Science & Technology, ChangSha 410 076, Hunan, P.R. China.;Department of Biology, Huaihua College, HuaiHua 418 000, Hunan, China.;Changsha Medical College, Changsha 410 219, PR China.
通讯机构：
Department of Biology, College of Chemistry and Biology, ChangSha University of Science and Technology, China
关键词：
Antibacterial activity;extract;Smilax china.
摘要：
The ethyl acetate extract, aqueous extract and membrane separation extract from the tubers of Smilax china were investigated for anti-bacterial and anti-fungal activity by agar plate disc-diffusion assay. All the three extracts showed anti-bacterial effects against all Gram-positive bacteria, Gram-negative bacteria and fungi tested. Among anti-bacterial activities of these extract, the ethyl acetate extracts exhibited stronger anti-microbial activity against Gram-positive bacteria as compared to aqueous extract and membrane separation extract showed similar to and even stronger anti-microbial activity than the aqueous extract, which may be that the former could possess higher concentration of the flavonoids and membrane separation extract possess higher concentration of the effective ingredients that prove that it is feasible that aqueous extract was further purified by membrane separation technique.
会议名称：
The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议)
会议时间：
2009-06-11
会议地点：
北京
会议论文集名称：
The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议)论文集
关键词：
Bioinformatics;DNA cloning;DNA fingerprint;DNA marker sequence;Gastrodia tuber;Genomic DNA sequence
摘要：
Gastrodia tuber has many pharmacological effects. DNA fingerprints of Gastrodia tuber were determined using randomly amplified polymorphic DNA (RAPD) technique, of which the desired DNA segments were selected and recovered. Positive DNA clones were obtained through ligation techniques, transformation, blue/white color screenings, cloning and identifying. Novelty of the cloned DNA and its potential importance were confirmed by sequencing DNA and bioinformatics analyzing. The results showed the DNA sequences were proven to be a new discovery, Sequence 1 and 3 had 3 and 1 Open Reading Frames (ORF), respectively. DNA sequence 2 have 13 ORFs, which can express 8 unintegrated function domains. The DNA sequence deserve further researching. The distribution of these 3 DNA sequences varied greatly among the populations, whereby DNA sequence 1 exist only in the populations that show the highest content of effective component gastrodin. The findings may be applied in exploiting genomic DNA and genetic classification of Gastrodia tuber populations.
摘要：
Aim of the study: Total flavonids of Polygonatum(P) odoratum (TFP) were tested for anti-diabetic activity in streptozotocin (STZ)-induced diabetic mice and alloxan-induced diabetic rats. Materials and methods: Rhizoma Polygonati Odorati, well-known Chinese traditional medicine, is widely used for treatment of diverse diseases for example diabetes. In our study,TFP was extracted by 70% ethanol and purified by macroreticular resin. The experiments were designed to detect the anti-diabetic activity of TFP by determination of blood glucose (BG) using one touch gluco-meter and insulin levels by using a radioimmunoassay kit in streptozotocin (STZ)-induced diabetic mice and alloxan-induced diabetic rats and alpha-amylase inhibitory activity by alpha-amylase inhibition assay in vitro. Results: TFP had beneficial effects on regulation of blood glucose. Daily administration with 50-200 mg/kg body weight of TFP for 9 days can reduce significantly hyperglycemia in STZ-induced diabetic mice. Thirtieth day administration with TFP (50-200 mg/kg body weight) also decreased significantly fasting blood glucose in alloxan-induced diabetic rats. The hypoglycemic effect of TFP at 50 and 100 mg/kg is less than that of acarbose 20 mg/kg and gliclazide 15 mg/kg. The hypoglycemic effects of TFP at 200 mg/kg is similar to that of acarbose 20 mg/kg and gliclazide 15 mg/kg. TFP also could increase significantly the insulin level in alloxan-induced type 2 diabetic rats (P < 0.05) compared with control. Alpha-amylase inhibition assay in vitro showed that TFP inhibited significantly alpha-amylase activity in a dose-dependent manner. Conclusions: TFP possess significant dose-dependent anti-diabetic activity. TFP is one of the primary hypoglycemic active compounds of Polygonatum odoratum which would worth further study and development. (C) 2009 Published by Elsevier Ireland Ltd.
关键词：
DNA polymorphism;Gastrodia elata Blume;Gastrodin;Genetic relationships;Chemical fingerprints
摘要：
Gastrodia tuber and its component gastrodin have many pharmacological effects. The chemical fingerprints and gastrodin contents of eight Gastrodia populations were determined, and the genomic DNA polymorphism of the populations was investigated. Genetic distance coefficients among the populations were calculated using the DNA polymorphism data. A dendrogram of the genetic similarities between the populations was constructed using the genetic distance coefficients. The results indicated that the genomic DNA of Gastrodia tubers was highly polymorphic; the eight populations clustered into three major groups, and the gastrodin content varied greatly among these groups. There were obvious correlations among genetic makeup, gastrodin content, and place of origin. The ecological environments in Guizhou and Shanxi may be conducive to evolution and to gastrodin biosynthesis, and more suitable for cultivation of Gastrodia tubers. These findings may provide a scientific basis for overall genetic resource management and for the selection of locations for cultivating Gastrodia tubers.
